Literature DB >> 18265198

Assembly of nucleosomal templates by salt dialysis.

K M Lee1, G Narlikar.   

Abstract

Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient), which are accompanied by protocols for purification of core histones from bacteria and preparation of DNA for the nucleosomal arrays. Electrophoretic procedures to analyze the reconstituted complexes are also included.

Mesh：

Substances：

Year:  2001        PMID： 18265198     DOI： 10.1002/0471142727.mb2106s54

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  19 in total

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4.  A role for BAF57 in cell cycle-dependent transcriptional regulation by the SWI/SNF chromatin remodeling complex.

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Journal:  Cancer Res       Date:  2010-05-11       Impact factor: 12.701

5.  Multiple sequence-specific factors generate the nucleosome-depleted region on CLN2 promoter.

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Journal:  Mol Cell       Date:  2011-05-20       Impact factor: 17.970

6.  A core subunit of Polycomb repressive complex 1 is broadly conserved in function but not primary sequence.

Authors:  Leslie Y Beh; Lucy J Colwell; Nicole J Francis
Journal:  Proc Natl Acad Sci U S A       Date:  2012-04-18       Impact factor: 11.205

Review 7.  Pioneer factors and their in vitro identification methods.

Authors:  Xinyang Yu; Michael J Buck
Journal:  Mol Genet Genomics       Date:  2020-04-15       Impact factor: 3.291

8.  Structure of the Human cGAS-DNA Complex Reveals Enhanced Control of Immune Surveillance.

Authors:  Wen Zhou; Aaron T Whiteley; Carina C de Oliveira Mann; Benjamin R Morehouse; Radosław P Nowak; Eric S Fischer; Nathanael S Gray; John J Mekalanos; Philip J Kranzusch
Journal:  Cell       Date:  2018-07-12       Impact factor: 41.582

9.  Monitoring conformational dynamics with single-molecule fluorescence energy transfer: applications in nucleosome remodeling.

Authors:  Sebastian Deindl; Xiaowei Zhuang
Journal:  Methods Enzymol       Date:  2012       Impact factor: 1.600

10.  Chromatin modification by PSC occurs at one PSC per nucleosome and does not require the acidic patch of histone H2A.

Authors:  Stanley M Lo; Kyle A McElroy; Nicole J Francis
Journal:  PLoS One       Date:  2012-10-11       Impact factor: 3.240
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