Literature DB >> 18264105

Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.

Micah Hamady1, Jeffrey J Walker, J Kirk Harris, Nicholas J Gold, Rob Knight.   

Abstract

We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.

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Year:  2008        PMID： 18264105      PMCID： PMC3439997          DOI： 10.1038/nmeth.1184

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  13 in total

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Authors:  Susan M Huse; Julie A Huber; Hilary G Morrison; Mitchell L Sogin; David Mark Welch
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