| Literature DB >> 18261893 |
Jieru Pan1, Tianpei Huang, Fan Yao, Zhipeng Huang, Charles A Powell, Sixin Qiu, Xiong Guan.
Abstract
AHL-lactonase (AiiA), a metallo-beta-lactamase produced by Bacillus thuringiensis, Bacillus cereus and Bacillus anthracis, specifically hydrolyzes N-acyl-homoserine lactones (AHLs) secreted by Gram-negative bacteria and thereby attenuates the symptoms caused by plant pathogens. In this study, an aiiA gene was cloned from Bacillus subtilis BS-1 by PCR with a pair of degenerate primers. The deduced 250 amino acid sequence contained two small conserved regions, 103SHLHFDH109 and 166TPGHTPGH173, which are characteristic of the metallo-beta-lactamase family. Homology comparison revealed that the deduced amino acid sequence had a high degree of similarity with those of the known AiiA proteins in the B. cereus group. Additionally, the aiiA gene was expressed in Escherichia coli BL21 (DE3) pLysS and the expressed AiiA protein could attenuate the soft rot symptoms caused by Erwinia carotovora var. carotovora.Entities:
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Year: 2008 PMID： 18261893 DOI： 10.1016/j.micres.2007.12.002
Source DB: PubMed Journal: Microbiol Res ISSN： 0944-5013 Impact factor: 5.415