| Literature DB >> 18248040 |
Y Y Fei1, J P Landry, Y S Sun, X D Zhu, J T Luo, X B Wang, K S Lam.
Abstract
We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.Mesh：
Year: 2008 PMID： 18248040 PMCID： PMC2715555 DOI： 10.1063/1.2830286
Source DB: PubMed Journal: Rev Sci Instrum ISSN： 0034-6748 Impact factor: 1.523