| Literature DB >> 18237722 |
Maria de Lourdes Corradi da Silva1, Eliane K Fukuda, Ana Flora D Vasconcelos, Robert F H Dekker, Andreza C Matias, Nilson K Monteiro, Marilsa S Cardoso, Aneli M Barbosa, Joana L M Silveira, Guilherme L Sassaki, Elaine R Carbonero.
Abstract
Three D-glucans were isolated from the mycelium of the fungus Botryosphaeria rhodina MAMB-05 by sequential extraction with hot-water and hot aqueous KOH (2% w/v) followed by ethanol precipitation. Following their purification by gel permeation chromatography on Sepharose CL-4B, the structural characteristics of the D-glucans were determined by FT-IR and 13C NMR spectroscopy and, after methylation, by GC-MS. The hot-water extract produced a fraction designated Q1A that was a beta-(1-->6)-D-glucan with the following structure: [Formula: see text] The alkaline extract, when subjected to repeated freeze-thawing, yielded two fractions: K1P (insoluble) that comprised a beta-(1-->3)-D-glucan with beta-D-glucose branches at C-6 with the structure: [Formula: see text] and K1SA (soluble) consisting of a backbone chain of alpha-(1-->4)-linked D-glucopyranosyl residues substituted at O-6 with alpha-D-glucopyranosyl residues: [Formula: see text]Entities:
Mesh：
Substances：
Year: 2008 PMID： 18237722 DOI： 10.1016/j.carres.2007.12.021
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104