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Literature DB >> 18228450

Preparation of human epidermal keratinocyte cultures.

Abstract

Cultured keratinocytes have been used by a number of investigators in studies investigating wound repair and carcinogenesis, and they have also proven useful as a model for differentiation. This unit describes a protocol for establishing human keratinocytes in tissue culture. Human newborn foreskins are proteolytically digested to separate the epidermis and the dermis, and keratinocytes are obtained from the epidermis.

Entities: Disease Species

Mesh：

Substances：
Culture Media
Trypsin

Year: 2004 PMID： 18228450 DOI： 10.1002/0471143030.cb0206s21

Source DB: PubMed Journal: Curr Protoc Cell Biol ISSN： 1934-2616

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Cited

4 in total

1. The Human Papillomavirus 16 E7 Oncoprotein Attenuates AKT Signaling To Promote Internal Ribosome Entry Site-Dependent Translation and Expression of c-MYC.

Authors: Sydney Webb Strickland; Scott Vande Pol
Journal: J Virol Date: 2016-05-27 Impact factor: 5.103

Preparation of human epidermal keratinocyte cultures.

1. The Human Papillomavirus 16 E7 Oncoprotein Attenuates AKT Signaling To Promote Internal Ribosome Entry Site-Dependent Translation and Expression of c-MYC.

2. Bioprinted Skin Recapitulates Normal Collagen Remodeling in Full-Thickness Wounds.

3. Evaluation of SAS1B as a target for antibody-drug conjugate therapy in the treatment of pancreatic cancer.

4. Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators.