Literature DB >> 18228339

Interaction trap/two-hybrid system to identify interacting proteins.

E A Golemis1, I Serebriiskii, R L Finley, M G Kolonin, J Gyuris, R Brent.   

Abstract

This unit presents protocols designed to detect interacting proteins. Using yeast as a "test tube" and transcriptional activation of a reporter system, interacting proteins can be identified. The system can also be used to test complex formation for proteins for which there exists a reason to expect interaction.

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Year:  2001        PMID： 18228339      PMCID： PMC4095973          DOI： 10.1002/0471143030.cb1703s08

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  30 in total

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Authors:  D Gietz; A St Jean; R A Woods; R H Schiestl
Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971

2.  Use of the two-hybrid system and random sonicated DNA to identify the interaction domain of a protein.

Authors:  I Stagljar; J P Bourquin; W Schaffner
Journal:  Biotechniques       Date:  1996-09       Impact factor: 1.993

3.  A three-hybrid system to detect RNA-protein interactions in vivo.

Authors:  D J SenGupta; B Zhang; B Kraemer; P Pochart; S Fields; M Wickens
Journal:  Proc Natl Acad Sci U S A       Date:  1996-08-06       Impact factor: 11.205

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Authors:  J Ma; M Ptashne
Journal:  Cell       Date:  1988-11-04       Impact factor: 41.582

5.  A two-hybrid dual bait system to discriminate specificity of protein interactions.

Authors:  I Serebriiskii; V Khazak; E A Golemis
Journal:  J Biol Chem       Date:  1999-06-11       Impact factor: 5.157

6.  A new class of yeast transcriptional activators.

Authors:  J Ma; M Ptashne
Journal:  Cell       Date:  1987-10-09       Impact factor: 41.582

7.  Protein-peptide interactions analyzed with the yeast two-hybrid system.

Authors:  M Yang; Z Wu; S Fields
Journal:  Nucleic Acids Res       Date:  1995-04-11       Impact factor: 16.971

8.  A novel genetic system to detect protein-protein interactions.

Authors:  S Fields; O Song
Journal:  Nature       Date:  1989-07-20       Impact factor: 49.962

9.  Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2.

Authors:  P Colas; B Cohen; T Jessen; I Grishina; J McCoy; R Brent
Journal:  Nature       Date:  1996-04-11       Impact factor: 49.962

10.  Enzymatic amplification of DNA by PCR: standard procedures and optimization.

Authors:  M F Kramer; D M Coen
Journal:  Curr Protoc Mol Biol       Date:  2001-11
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  6 in total

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6.  Calcitonin Receptor-Zonula Occludens-1 Interaction Is Critical for Calcitonin-Stimulated Prostate Cancer Metastasis.

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  6 in total

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