Literature DB >> 18226423

Employing phage display to study the mode of action of Bacillus thuringiensis Cry toxins.

Luisa Elena Fernández1, Isabel Gómez, Sabino Pacheco, Iván Arenas, Sarjeet S Gilla, Alejandra Bravo, Mario Soberón.   

Abstract

Phage display is an in vitro method for selecting polypeptides with desired properties from a large collection of variants. The insecticidal Cry toxins produced by Bacillus thuringiensis are highly specific to different insects. Various proteins such as cadherin, aminopeptidase-N (APN) and alkaline phosphatase (ALP) have been characterized as potential Cry-receptors. We used phage display to characterize the Cry toxin-receptor interaction(s). By employing phage-libraries that display single-chain antibodies (scFv) from humans or from immunized rabbits with Cry1Ab toxin or random 12-residues peptides, we have identified the epitopes that mediate binding of lepidopteran Cry1Ab toxin with cadherin and APN receptors from Manduca sexta and the interaction of dipteran Cry11Aa toxin with the ALP receptor from Aedes aegypti. Finally we displayed in phages the Cry1Ac toxin and discuss the potential for selecting Cry variants with improved toxicity or different specificity.

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Year:  2007        PMID： 18226423      PMCID： PMC2267870          DOI： 10.1016/j.peptides.2007.07.035

Source DB:  PubMed          Journal:  Peptides        ISSN： 0196-9781            Impact factor:   3.750


  33 in total

1.  Cadherin-like receptor binding facilitates proteolytic cleavage of helix alpha-1 in domain I and oligomer pre-pore formation of Bacillus thuringiensis Cry1Ab toxin.

Authors:  Isabel Gómez; Jorge Sánchez; Raúl Miranda; Alejandra Bravo; Mario Soberón
Journal:  FEBS Lett       Date:  2002-02-27       Impact factor: 4.124

Review 2.  Convergent evolution with combinatorial peptides.

Authors:  B K Kay; J Kasanov; S Knight; A Kurakin
Journal:  FEBS Lett       Date:  2000-08-25       Impact factor: 4.124

Review 3.  Phage display technology: clinical applications and recent innovations.

Authors:  Hassan M E Azzazy; W Edward Highsmith
Journal:  Clin Biochem       Date:  2002-09       Impact factor: 3.281

4.  Molecular basis for Bacillus thuringiensis Cry1Ab toxin specificity: two structural determinants in the Manduca sexta Bt-R1 receptor interact with loops alpha-8 and 2 in domain II of Cy1Ab toxin.

Authors:  Isabel Gómez; Donald H Dean; Alejandra Bravo; Mario Soberón
Journal:  Biochemistry       Date:  2003-09-09       Impact factor: 3.162

5.  Structure of the insecticidal bacterial delta-endotoxin Cry3Bb1 of Bacillus thuringiensis.

Authors:  N Galitsky; V Cody; A Wojtczak; D Ghosh; J R Luft; W Pangborn; L English
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2001-07-23

Review 6.  Biochemistry and genetics of insect resistance to Bacillus thuringiensis.

Authors:  Juan Ferré; Jeroen Van Rie
Journal:  Annu Rev Entomol       Date:  2002       Impact factor: 19.686

7.  Characterization of a Cry1Ac-receptor alkaline phosphatase in susceptible and resistant Heliothis virescens larvae.

Authors:  Juan L Jurat-Fuentes; Michael J Adang
Journal:  Eur J Biochem       Date:  2004-08

8.  Hydropathic complementarity determines interaction of epitope (869)HITDTNNK(876) in Manduca sexta Bt-R(1) receptor with loop 2 of domain II of Bacillus thuringiensis Cry1A toxins.

Authors:  Isabel Gomez; Juan Miranda-Rios; Enrique Rudiño-Piñera; Daniela I Oltean; Sarjeet S Gill; Alejandra Bravo; Mario Soberón
Journal:  J Biol Chem       Date:  2002-06-05       Impact factor: 5.157

9.  Tryptophan spectroscopy studies and black lipid bilayer analysis indicate that the oligomeric structure of Cry1Ab toxin from Bacillus thuringiensis is the membrane-insertion intermediate.

Authors:  Carolina Rausell; Carlos Muñoz-Garay; Raúl Miranda-CassoLuengo; Isabel Gómez; Enrique Rudiño-Piñera; Mario Soberón; Alejandra Bravo
Journal:  Biochemistry       Date:  2004-01-13       Impact factor: 3.162

10.  Binding of phage displayed Bacillus subtilis lipase A to a phosphonate suicide inhibitor.

Authors:  Melloney J Dröge; Carsten J Rüggeberg; Almer M van der Sloot; Judith Schimmel; Dolf Swaving Dijkstra; Raymond M D Verhaert; Manfred T Reetz; Wim J Quax
Journal:  J Biotechnol       Date:  2003-02-27       Impact factor: 3.307
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  4 in total

1.  Evaluation of seed extracts from plants found in the Caatinga biome for the control of Aedes aegypti.

Authors:  Patrícia Batista Barra Medeiros Barbosa; Julliete Medeiros de Oliveira; Juliana Macêdo Chagas; Luciana Maria Araujo Rabelo; Guilherme Fulgêncio de Medeiros; Raquel Brant Giodani; Elizeu Antunes da Silva; Adriana Ferreira Uchôa; Maria de Fátima de Freire Melo Ximenes
Journal:  Parasitol Res       Date:  2014-07-24       Impact factor: 2.289

2.  Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis).

Authors:  Gustavo R Oliveira; Maria C M Silva; Wagner A Lucena; Erich Y T Nakasu; Alexandre A P Firmino; Magda A Beneventi; Djair S L Souza; José E Gomes; José D A de Souza; Daniel J Rigden; Hudson B Ramos; Carlos R Soccol; Maria F Grossi-de-Sa
Journal:  BMC Biotechnol       Date:  2011-09-09       Impact factor: 2.563

3.  Affinity maturation of Cry1Aa toxin to the Bombyx mori cadherin-like receptor by directed evolution based on phage display and biopanning selections of domain II loop 2 mutant toxins.

Authors:  Haruka Endo; Yuki Kobayashi; Yasushi Hoshino; Shiho Tanaka; Shingo Kikuta; Hiroko Tabunoki; Ryoichi Sato
Journal:  Microbiologyopen       Date:  2014-07-16       Impact factor: 3.139

4.  Weight of the evidence: independent research projects confirm industry conclusions on the safety of insect-protected maize MON 810.

Authors:  Jay S Petrick; Erin Bell; Michael S Koch
Journal:  GM Crops Food       Date:  2019-10-25       Impact factor: 3.074
  4 in total

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