BACKGROUND: Histone deacetylase (HDAC) inhibitors have anticancer effects. Their effects on expression of cell adhesion molecules might be related to their effects on tumor cell invasion. MATERIALS AND METHODS: Murine B16-BL6 cells were treated with the HDAC inhibitors, butyrate or trichostatin A. Melanoma cell invasion of the artificial basement membrane, Matrigel, was examined by Transwell chamber assay. RESULTS: Butyrate as well as trichostatin A inhibited the cell growth mainly by arresting the cell cycle. The cell invasion of Matrigel was inhibited by butyrate and trichostatin A. The butyrate treatment increased the cell-cell aggregation, although neither E-cadherin nor N-cadherin mRNA were up-regulated. Both mRNA expression and protein levels of the immunoglobulin superfamily cell adhesion molecules, Mel-CAM and L1-CAM, were increased in the butyrate-treated cells. CONCLUSION: The HDAC inhibitor butyrate blocked the B16-BL6 melanoma cell invasion of Matrigel, although it increased the expression of Mel-CAM and L1-CAM which are important to the metastatic potential.
BACKGROUND: Histone deacetylase (HDAC) inhibitors have anticancer effects. Their effects on expression of cell adhesion molecules might be related to their effects on tumor cell invasion. MATERIALS AND METHODS:Murine B16-BL6 cells were treated with the HDAC inhibitors, butyrate or trichostatin A. Melanoma cell invasion of the artificial basement membrane, Matrigel, was examined by Transwell chamber assay. RESULTS:Butyrate as well as trichostatin A inhibited the cell growth mainly by arresting the cell cycle. The cell invasion of Matrigel was inhibited by butyrate and trichostatin A. The butyrate treatment increased the cell-cell aggregation, although neither E-cadherin nor N-cadherin mRNA were up-regulated. Both mRNA expression and protein levels of the immunoglobulin superfamily cell adhesion molecules, Mel-CAM and L1-CAM, were increased in the butyrate-treated cells. CONCLUSION: The HDAC inhibitor butyrate blocked the B16-BL6 melanoma cell invasion of Matrigel, although it increased the expression of Mel-CAM and L1-CAM which are important to the metastatic potential.
Authors: Uwe Schirmer; Heidi Fiegl; Marco Pfeifer; Alain G Zeimet; Elisabeth Müller-Holzner; Peter K Bode; Verena Tischler; Peter Altevogt Journal: BMC Cancer Date: 2013-03-26 Impact factor: 4.430
Authors: Uwe Schirmer; Kai Doberstein; Anne-Kathleen Rupp; Niko P Bretz; Daniela Wuttig; Helena Kiefel; Christian Breunig; Heidi Fiegl; Elisabeth Müller-Holzner; Robert Zeillinger; Eva Schuster; Alain G Zeimet; Holger Sültmann; Peter Altevogt Journal: Oncotarget Date: 2014-01-30
Authors: María Díaz-Núñez; Alejandro Díez-Torre; Olivier De Wever; Ricardo Andrade; Jon Arluzea; Margarita Silió; Juan Aréchaga Journal: BMC Cancer Date: 2016-08-22 Impact factor: 4.430