BACKGROUND: Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which are most abundant in the liver, are the main enzymes involved in ethanol and acetaldehyde metabolism. AIMS: The purpose of this study was to compare the activity of ADH isoenzymes and ALDH between liver carcinoma cells and healthy hepatocytes. PATIENTS: Samples were taken from 44 liver cancer patients (19 drinkers, 25 nondrinkers). Seventeen patients had primary liver tumors and 27 had metastatic liver tumors. METHODS: Fluorimetric methods were used for measurement of the activity of class I and II ADH isoenzymes and ALDH activity. Total ADH activity and activity of class III and IV isoenzymes were measured by a photometric method. RESULTS: The activities of total ADH, ALDH, and class I ADH were significantly higher in cancer cells than in healthy tissues. The other tested classes of ADH showed a tendency toward higher activity in cancer than in normal cells. The differences between enzymes of drinkers and nondrinkers in both cancer and healthy tissue, were not significant. CONCLUSION: Differences in the activities of total ADH, ALDH and class I ADH isoenzyme between cancer liver tissues and healthy hepatocytes might be a factor in ethanol metabolism disorders, which can intensify carcinogenesis.
BACKGROUND:Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which are most abundant in the liver, are the main enzymes involved in ethanol and acetaldehyde metabolism. AIMS: The purpose of this study was to compare the activity of ADH isoenzymes and ALDH between liver carcinoma cells and healthy hepatocytes. PATIENTS: Samples were taken from 44 liver cancerpatients (19 drinkers, 25 nondrinkers). Seventeen patients had primary liver tumors and 27 had metastatic liver tumors. METHODS: Fluorimetric methods were used for measurement of the activity of class I and II ADH isoenzymes and ALDH activity. Total ADH activity and activity of class III and IV isoenzymes were measured by a photometric method. RESULTS: The activities of total ADH, ALDH, and class I ADH were significantly higher in cancer cells than in healthy tissues. The other tested classes of ADH showed a tendency toward higher activity in cancer than in normal cells. The differences between enzymes of drinkers and nondrinkers in both cancer and healthy tissue, were not significant. CONCLUSION: Differences in the activities of total ADH, ALDH and class I ADH isoenzyme between cancer liver tissues and healthy hepatocytes might be a factor in ethanolmetabolism disorders, which can intensify carcinogenesis.
Authors: Magdalena Laniewska-Dunaj; Wojciech Jelski; Karolina Orywal; Jan Kochanowicz; Robert Rutkowski; Maciej Szmitkowski Journal: Neurochem Res Date: 2013-04-27 Impact factor: 3.996
Authors: Wojciech Jelski; Magdalena Laniewska-Dunaj; Karolina Orywal; Jan Kochanowicz; Robert Rutkowski; Maciej Szmitkowski Journal: Neurochem Res Date: 2014-10-10 Impact factor: 3.996