BACKGROUND AND PURPOSE: The management of lymphatic metastasis of early-stage oral tongue carcinoma patients is crucial for its prognosis. The purpose of this study was to evaluate the predictive ability of lymphatic metastasis after brachytherapy (BRT) for early-stage tongue carcinoma based on gene expression profiling. PATIENTS AND METHODS: Pre-therapeutic biopsies from 39 patients with T1 or T2 tongue cancer were analyzed for gene expression signatures using Codelink Uniset Human 20K Bioarray. All patients were treated with low dose-rate BRT for their primary lesions and underwent strict follow-up under a wait-and-see policy for cervical lymphatic metastasis. Candidate genes were selected for predicting lymph-node status in the reference group by the permutation test. Predictive accuracy was further evaluated by the prediction strength (PS) scoring system using an independent validation group. RESULTS: We selected a set of 19 genes whose expression differed significantly between classes with or without lymphatic metastasis in the reference group. The lymph-node status in the validation group was predicted by the PS scoring system with an accuracy of 76%. CONCLUSIONS: Gene expression profiling using 19 genes in primary tumor tissues may allow prediction of lymphatic metastasis after BRT for early-stage oral tongue carcinoma.
BACKGROUND AND PURPOSE: The management of lymphatic metastasis of early-stage oral tongue carcinomapatients is crucial for its prognosis. The purpose of this study was to evaluate the predictive ability of lymphatic metastasis after brachytherapy (BRT) for early-stage tongue carcinoma based on gene expression profiling. PATIENTS AND METHODS: Pre-therapeutic biopsies from 39 patients with T1 or T2 tongue cancer were analyzed for gene expression signatures using Codelink Uniset Human 20K Bioarray. All patients were treated with low dose-rate BRT for their primary lesions and underwent strict follow-up under a wait-and-see policy for cervical lymphatic metastasis. Candidate genes were selected for predicting lymph-node status in the reference group by the permutation test. Predictive accuracy was further evaluated by the prediction strength (PS) scoring system using an independent validation group. RESULTS: We selected a set of 19 genes whose expression differed significantly between classes with or without lymphatic metastasis in the reference group. The lymph-node status in the validation group was predicted by the PS scoring system with an accuracy of 76%. CONCLUSIONS: Gene expression profiling using 19 genes in primary tumor tissues may allow prediction of lymphatic metastasis after BRT for early-stage oral tongue carcinoma.
Authors: Xifeng Wu; Yuanqing Ye; Rafael Rosell; Christopher I Amos; David J Stewart; Michelle A T Hildebrandt; Jack A Roth; John D Minna; Jian Gu; Jie Lin; Shama C Buch; Tomoko Nukui; Jose Luis Ramirez Serrano; Miquel Taron; Adrian Cassidy; Charles Lu; Joe Y Chang; Scott M Lippman; Waun Ki Hong; Margaret R Spitz; Marjorie Romkes; Ping Yang Journal: J Natl Cancer Inst Date: 2011-04-11 Impact factor: 13.506
Authors: Lubos Tuček; Jiri Petera; Igor Sirák; Milan Vošmik; Helena Doležalová; Simona Brokešová; Miroslav Hodek; Linda Kašaová; Petr Paluska Journal: Rep Pract Oncol Radiother Date: 2011-08-06