Literature DB >> 18221003

Expression and purification of CB2 for NMR studies in micellar solution.

Dmitriy Krepkiy1, Klaus Gawrisch, Alexei Yeliseev.   

Abstract

We demonstrate feasibility of biophysical characterization of the peripheral cannabinoid receptor CB2 produced by heterologous expression in E. coli membranes. Recombinant receptor was purified by affinity chromatography, and NMR diffusion experiments performed on CB2 solubilized in dodecylphosphocholine (DPC) micelles. Circular dichroism spectroscopy indicated high alpha-helical content (49 %) of CB2.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 18221003     DOI： 10.2174/092986607782541051

Source DB:  PubMed          Journal:  Protein Pept Lett        ISSN： 0929-8665            Impact factor:   1.890


  7 in total

1.  Mistic and TarCF as fusion protein partners for functional expression of the cannabinoid receptor 2 in Escherichia coli.

Authors:  Ananda Chowdhury; Rentian Feng; Qin Tong; Yuxun Zhang; Xiang-Qun Xie
Journal:  Protein Expr Purif       Date:  2012-03-03       Impact factor: 1.650

2.  Global fold of human cannabinoid type 2 receptor probed by solid-state 13C-, 15N-MAS NMR and molecular dynamics simulations.

Authors:  Tomohiro Kimura; Krishna Vukoti; Diane L Lynch; Dow P Hurst; Alan Grossfield; Michael C Pitman; Patricia H Reggio; Alexei A Yeliseev; Klaus Gawrisch
Journal:  Proteins       Date:  2013-10-17

3.  Probing thermostability of detergent-solubilized CB2 receptor by parallel G protein-activation and ligand-binding assays.

Authors:  Ryan L Beckner; Lioudmila Zoubak; Kirk G Hines; Klaus Gawrisch; Alexei A Yeliseev
Journal:  J Biol Chem       Date:  2019-11-27       Impact factor: 5.157

4.  Recombinant cannabinoid type 2 receptor in liposome model activates g protein in response to anionic lipid constituents.

Authors:  Tomohiro Kimura; Alexei A Yeliseev; Krishna Vukoti; Steven D Rhodes; Kejun Cheng; Kenner C Rice; Klaus Gawrisch
Journal:  J Biol Chem       Date:  2011-12-01       Impact factor: 5.157

5.  Advances in methods to characterize ligand-induced ionic lock and rotamer toggle molecular switch in G protein-coupled receptors.

Authors:  Xiang-Qun Xie; Ananda Chowdhury
Journal:  Methods Enzymol       Date:  2013       Impact factor: 1.600

6.  Use of tandem affinity chromatography for purification of cannabinoid receptor CB₂.

Authors:  Silvia C Locatelli-Hoops; Alexei A Yeliseev
Journal:  Methods Mol Biol       Date:  2014

Review 7.  Methods for recombinant expression and functional characterization of human cannabinoid receptor CB2.

Authors:  Alexei A Yeliseev
Journal:  Comput Struct Biotechnol J       Date:  2013-09-06       Impact factor: 7.271
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.