| Literature DB >> 18208401 |
Alessandro Costa1, Silvia Onesti.
Abstract
The MCM2-MCM7 (minichromosome maintenance 2-7) complex is involved both in the initiation and the elongation step of eukaryotic DNA replication and is believed to be the replicative helicase. Whereas the mechanism of DNA unwinding at the replication fork has been extensively investigated, the role of the MCM2-MCM7 complex during initiation has not yet been characterized by biochemical studies. Here we summarize the in vivo evidence which supports a role for the MCM complex in origin melting. In addition, we present an overview of the mechanism of action of a number of AAA+ (ATPase associated with various cellular activities) initiators and hexameric helicases, which can be used in turn as models for the steps of recognition, duplex melting, loading and nucleic acid translocation of the MCM helicase.Entities:
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Year: 2008 PMID： 18208401 DOI： 10.1042/BST0360136
Source DB: PubMed Journal: Biochem Soc Trans ISSN： 0300-5127 Impact factor: 5.407