Chi-Dan Wan1, Chun-You Wang, Tao Liu, Rui Cheng, Hong-Bo Wang. 1. Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. chidanwan@163.com
Abstract
AIM: To investigate the protective effect of target suppression of uncoupling protein-2 (UCP-2) on ischemia/reperfusion (I/R) injury in fatty liver in ob/ob mice. METHODS: Plasmids suppressing UCP-2 expression were constructed, and transfected into fatty liver cells cultured in vitro and the ob/ob mouse I/R injury model. Serum tumor necrosis factor (TNF)- alpha levels, UCP-2 mRNA expression, alanine aminotransferase (ALT) levels in ob/ob mice were tested, and the pathological changes in fatty liver were observed in experimental and control groups. RESULTS: In ob/ob mouse I/R models, serum TNF-alpha levels were significantly higher than in normal controls. After the plasmids were transfected into the cultured cells and animal models, expression of UCP-2 mRNA was significantly reduced as compared with that in the control group (2(1.56+/-0.15) vs 2(-0.45+/-0.15), P<0.05). In ob/ob mouse models, in which expression of UCP-2 was suppressed, serum ALT levels were significantly lower than those of other groups, and pathological analysis revealed that injury of liver tissues was significantly alleviated. CONCLUSION: The target suppression of UCP-2 expression in fatty liver can alleviate the I/R injury in the ob/ob mice.
AIM: To investigate the protective effect of target suppression of uncoupling protein-2 (UCP-2) on ischemia/reperfusion (I/R) injury in fatty liver in ob/ob mice. METHODS: Plasmids suppressing UCP-2 expression were constructed, and transfected into fatty liver cells cultured in vitro and the ob/ob mouse I/R injury model. Serum tumor necrosis factor (TNF)- alpha levels, UCP-2 mRNA expression, alanine aminotransferase (ALT) levels in ob/ob mice were tested, and the pathological changes in fatty liver were observed in experimental and control groups. RESULTS: In ob/ob mouse I/R models, serum TNF-alpha levels were significantly higher than in normal controls. After the plasmids were transfected into the cultured cells and animal models, expression of UCP-2 mRNA was significantly reduced as compared with that in the control group (2(1.56+/-0.15) vs 2(-0.45+/-0.15), P<0.05). In ob/ob mouse models, in which expression of UCP-2 was suppressed, serum ALT levels were significantly lower than those of other groups, and pathological analysis revealed that injury of liver tissues was significantly alleviated. CONCLUSION: The target suppression of UCP-2 expression in fatty liver can alleviate the I/R injury in the ob/ob mice.
Authors: A Nègre-Salvayre; C Hirtz; G Carrera; R Cazenave; M Troly; R Salvayre; L Pénicaud; L Casteilla Journal: FASEB J Date: 1997-08 Impact factor: 5.191
Authors: K Murakami; F Kobayashi; R Ikegawa; M Koyama; N Shintani; T Yoshida; N Nakamura; T Kondo Journal: Eur J Pharmacol Date: 1998-01-02 Impact factor: 4.432
Authors: C B Chan; D De Leo; J W Joseph; T S McQuaid; X F Ha; F Xu; R G Tsushima; P S Pennefather; A M Salapatek; M B Wheeler Journal: Diabetes Date: 2001-06 Impact factor: 9.461
Authors: Amit D Tevar; Callisia N Clarke; Rebecca Schuster; Jiang Wang; Michael J Edwards; Alex B Lentsch Journal: J Surg Res Date: 2011-03-02 Impact factor: 2.192
Authors: Alton G Sutter; Arun P Palanisamy; Justin D Ellet; Michael G Schmidt; Rick G Schnellmann; Kenneth D Chavin Journal: Eur Cytokine Netw Date: 2014 Oct-Dec Impact factor: 2.737
Authors: Arnau Panisello Rosello; Rui Teixeira da Silva; Carlos Castro; Raquel G Bardallo; Maria Calvo; Emma Folch-Puy; Teresa Carbonell; Carlos Palmeira; Joan Roselló Catafau; René Adam Journal: Int J Mol Sci Date: 2020-08-09 Impact factor: 5.923