PURPOSES: The number of patients with nonalcoholic fatty liver disease (NAFLD) is increasing. Hepatic steatosis is a major risk factor for hepatic failure after ischemia-reperfusion (I/R) injury. Hydrolyzed whey peptide (HWP) is a functional liquid-type nutritional diet containing whey peptide, which has previously been shown to exert anti-inflammatory effects. In the present study, we examined the effects of HWP on the hepatic I/R injury in a rat NAFLD model. METHODS: Rats fed a methionine/choline-deficient diet for 4 weeks were divided into two groups after 30 min of whole liver ischemia. In Group-M, HWP was given immediately after reperfusion and every 6 h thereafter. In Group-C, the vehicle was given in the same manner. The liver function tests and microscopic findings of the liver after reperfusion were compared between the two groups. RESULTS: The serum transaminase levels in Group-M were significantly lower than those in Group-C after reperfusion. The gene expression levels of IL-6 and inducible nitric oxide synthase (iNOS) were significantly lower in Group-M compared to Group-C. The TNF-α and uncoupling protein-2 (UCP-2) expression levels were also markedly lower in Group-M. The hepatic necrotic areas in Group-M were significantly smaller than those in Group-C. CONCLUSION: The administration of a HWP diet ameliorated the hepatic I/R injury in rats with NAFLD.
PURPOSES: The number of patients with nonalcoholic fatty liver disease (NAFLD) is increasing. Hepatic steatosis is a major risk factor for hepatic failure after ischemia-reperfusion (I/R) injury. Hydrolyzed whey peptide (HWP) is a functional liquid-type nutritional diet containing whey peptide, which has previously been shown to exert anti-inflammatory effects. In the present study, we examined the effects of HWP on the hepatic I/R injury in a rat NAFLD model. METHODS:Rats fed a methionine/choline-deficient diet for 4 weeks were divided into two groups after 30 min of whole liver ischemia. In Group-M, HWP was given immediately after reperfusion and every 6 h thereafter. In Group-C, the vehicle was given in the same manner. The liver function tests and microscopic findings of the liver after reperfusion were compared between the two groups. RESULTS: The serum transaminase levels in Group-M were significantly lower than those in Group-C after reperfusion. The gene expression levels of IL-6 and inducible nitric oxide synthase (iNOS) were significantly lower in Group-M compared to Group-C. The TNF-α and uncoupling protein-2 (UCP-2) expression levels were also markedly lower in Group-M. The hepatic necrotic areas in Group-M were significantly smaller than those in Group-C. CONCLUSION: The administration of a HWP diet ameliorated the hepatic I/R injury in rats with NAFLD.
Authors: Mingyan Zhou; Aimin Xu; Paul K H Tam; Karen S L Lam; Lawrence Chan; Ruby L C Hoo; Jing Liu; Kim H M Chow; Yu Wang Journal: Hepatology Date: 2008-10 Impact factor: 17.425
Authors: Y Takeda; S Arii; T Kaido; M Niwano; T Moriga; A Mori; K Hanaki; M J Gorrin-Rivas; T Ishii; M Sato; M Imamura Journal: Transplantation Date: 1999-03-27 Impact factor: 4.939
Authors: M E Rinella; E Alonso; S Rao; P Whitington; J Fryer; M Abecassis; R Superina; S L Flamm; A T Blei Journal: Liver Transpl Date: 2001-05 Impact factor: 5.799
Authors: Richard Kirsch; Vivian Clarkson; Enid G Shephard; David A Marais; Mohamed A Jaffer; Vivienne E Woodburne; Ralph E Kirsch; Pauline de la M Hall Journal: J Gastroenterol Hepatol Date: 2003-11 Impact factor: 4.029
Authors: David A Kooby; Yuman Fong; Arief Suriawinata; Mithat Gonen; Peter J Allen; David S Klimstra; Ronald P DeMatteo; Michael D'Angelica; Leslie H Blumgart; William R Jarnagin Journal: J Gastrointest Surg Date: 2003-12 Impact factor: 3.267
Authors: María Eugenia Cornide-Petronio; Ana Isabel Álvarez-Mercado; Mónica B Jiménez-Castro; Carmen Peralta Journal: Nutrients Date: 2020-01-21 Impact factor: 5.717