BACKGROUND: Circulating procoagulant microparticles are reliable markers of vascular damage. The microparticle phenotypes provide additional information reflecting the nature of cell injury. This study assessed procoagulant microparticle levels and phenotypes in the diagnosis of acute allograft rejection after heart transplantation. METHODS: Microparticles were prospectively investigated in the venous blood of 64 heart transplant patients, 23 with allograft rejection mainly of low score, and 41 without a rejection episode. Plasma concentrations of cytokines, cytoadhesins, and platelet activation markers were determined. RESULTS: By univariate analysis, the mean time elapsed from heart transplant, cold ischemia time, E-selectin-, Fas- and tissue factor-bearing microparticles were associated with allograft rejection. By multivariate analysis, E-selectin-microparticle levels appeared independently associated with allograft rejection, even when other significant variables were included in the model (odds ratio, 9.8; 95% confidence interval, 1.36-71.4; p = 0.023). CONCLUSION: The pattern of procoagulant microparticles released during acute allograft rejection suggests endothelial cell activation and Fas-mediated apoptosis. E-selectin-bearing microparticles appeared as an independent marker of acute allograft rejection that was still informative after adjustment for graft characteristics.
BACKGROUND: Circulating procoagulant microparticles are reliable markers of vascular damage. The microparticle phenotypes provide additional information reflecting the nature of cell injury. This study assessed procoagulant microparticle levels and phenotypes in the diagnosis of acute allograft rejection after heart transplantation. METHODS: Microparticles were prospectively investigated in the venous blood of 64 heart transplant patients, 23 with allograft rejection mainly of low score, and 41 without a rejection episode. Plasma concentrations of cytokines, cytoadhesins, and platelet activation markers were determined. RESULTS: By univariate analysis, the mean time elapsed from heart transplant, cold ischemia time, E-selectin-, Fas- and tissue factor-bearing microparticles were associated with allograft rejection. By multivariate analysis, E-selectin-microparticle levels appeared independently associated with allograft rejection, even when other significant variables were included in the model (odds ratio, 9.8; 95% confidence interval, 1.36-71.4; p = 0.023). CONCLUSION: The pattern of procoagulant microparticles released during acute allograft rejection suggests endothelial cell activation and Fas-mediated apoptosis. E-selectin-bearing microparticles appeared as an independent marker of acute allograft rejection that was still informative after adjustment for graft characteristics.
Authors: Anne M Curtis; Lifeng Zhang; Elizabeth Medenilla; Ming Gui; Patrick F Wilkinson; Erding Hu; Jay Giri; Vijay Doraiswamy; Sampath Gunda; Mark E Burgert; Jonni S Moore; Jay M Edelberg; Emile R Mohler Journal: Cytometry B Clin Cytom Date: 2010-06-11 Impact factor: 3.058
Authors: Keely L Buesing; John C Densmore; Sushma Kaul; Kirkwood A Pritchard; Jason A Jarzembowski; David M Gourlay; Keith T Oldham Journal: J Surg Res Date: 2010-06-09 Impact factor: 2.192
Authors: Anne M Curtis; Jay Edelberg; Rebecca Jonas; Wade T Rogers; Jonni S Moore; Wajihuddin Syed; Emile R Mohler Journal: Vasc Med Date: 2013-07-26 Impact factor: 3.239
Authors: Iwijn De Vlaminck; Hannah A Valantine; Thomas M Snyder; Calvin Strehl; Garrett Cohen; Helen Luikart; Norma F Neff; Jennifer Okamoto; Daniel Bernstein; Dana Weisshaar; Stephen R Quake; Kiran K Khush Journal: Sci Transl Med Date: 2014-06-18 Impact factor: 17.956
Authors: Abdelhafeez H Abdelhafeez; Paul M Jeziorczak; Terry R Schaid; Susan L Hoefs; Sushma Kaul; Rahul Nanchal; Elizabeth R Jacobs; John C Densmore Journal: J Extracell Vesicles Date: 2014-02-27