| Literature DB >> 18164030 |
Anselm G Kusser1, Michela G Bertero, Souad Naji, Thomas Becker, Michael Thomm, Roland Beckmann, Patrick Cramer.
Abstract
Related multisubunit RNA polymerases (RNAPs) carry out gene transcription in all kingdoms of life. Since structural information is limited to bacterial and eukaryotic RNAPs, we determined the cryo-electron microscopic structure of the RNAP from the thermophilic archaeon Pyrococcus furiosus at 13 A resolution. Comparison with eukaryotic RNAP II reveals a conserved architecture, no homologues for subunits Rpb8 and Rpb9, and significant deviation in the polymerase foot, jaws, pore, and protrusion. The structural organization of the archaeal RNA polymerase serves as a reference for future structure-function analysis of the transcription mechanism and allows for evolutionary comparisons.Mesh：
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Year: 2007 PMID： 18164030 DOI： 10.1016/j.jmb.2007.08.066
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469