OBJECTIVE: This study investigated the effect of photodynamic therapy (PDT) with toluidine blue O (TBO) and a light-emitting diode (LED) on the viability of Streptococcus mutans cells in a planktonic culture. BACKGROUND DATA: Growth of Streptococcus mutans is the first step in the development of tooth decay. The use of light and dyes promotes cellular death in a noninvasive way, reducing treatment time. METHODS: The LED used in this study had output power of 116 mW, its energy was 21 J, and the fluency was 2.18 J/cm(2). Samples were prepared and divided into five groups: (1) control group (-); (2) control group (+); (3) TBO; (4) LED; and (5) LED + TBO. RESULTS: One hundred percent of the bacteria were killed following irradiation with LED and TBO. The biofilm that formed on the glass surfaces was analyzed by SEM and colony count. CONCLUSIONS: It was demonstrated that PDT was efficient at killing microorganisms and preventing the formation of biofilms.
OBJECTIVE: This study investigated the effect of photodynamic therapy (PDT) with toluidine blue O (TBO) and a light-emitting diode (LED) on the viability of Streptococcus mutans cells in a planktonic culture. BACKGROUND DATA: Growth of Streptococcus mutans is the first step in the development of tooth decay. The use of light and dyes promotes cellular death in a noninvasive way, reducing treatment time. METHODS: The LED used in this study had output power of 116 mW, its energy was 21 J, and the fluency was 2.18 J/cm(2). Samples were prepared and divided into five groups: (1) control group (-); (2) control group (+); (3) TBO; (4) LED; and (5) LED + TBO. RESULTS: One hundred percent of the bacteria were killed following irradiation with LED and TBO. The biofilm that formed on the glass surfaces was analyzed by SEM and colony count. CONCLUSIONS: It was demonstrated that PDT was efficient at killing microorganisms and preventing the formation of biofilms.
Authors: Martin Schneider; Gregor Kirfel; Michael Berthold; Matthias Frentzen; Felix Krause; Andreas Braun Journal: Lasers Med Sci Date: 2011-10-02 Impact factor: 3.161
Authors: Carlos de Paula Eduardo; Patricia Moreira de Freitas; Marcella Esteves-Oliveira; Ana Cecília Corrêa Aranha; Karen Müller Ramalho; Alyne Simões; Marina Stella Bello-Silva; Jan Tunér Journal: Lasers Med Sci Date: 2010-07-17 Impact factor: 3.161
Authors: João Paulo F Longo; Soraya C Leal; Andreza R Simioni; Maria de Fátima Menezes Almeida-Santos; Antônio C Tedesco; Ricardo B Azevedo Journal: Lasers Med Sci Date: 2011-08-02 Impact factor: 3.161