BACKGROUND: Rodents have been used to examine physiologic changes after bariatric surgery, but differences in gastric/vagal anatomy may limit their utility. Swine may be a more appropriate animal model because of anatomic and physiologic similarities to humans. The aim of this study was to establish a survival model of Roux-en-Y gastric bypass (RYGBP) in swine and to evaluate its potential in studies of physiology. METHODS: 13 miniature swine, 5 Yucatan [26.4 +/- 1.6 kg], 4 Hanford [28.3 +/- 0.6 kg] and 4 other breed [54.9 +/- 6.2 kg] underwent open RYGBP, and were kept alive to 30 (n=4), 60 (n=1) or 90 (n=2) postoperative days. RESULTS: 4 early animals had staple-line leakage within 7 days from surgery and 1 animal experienced unmanageable pain at 42 days after surgery. One animal experienced immediate cardiopulmonary collapse. 58% of animals survived to their projected endpoint. Necropsy of 1 animal at its 90-day endpoint revealed a gastro-gastric fistula. Anatomic features in swine that differ from humans, such as thick perigastric membranes, required adjustment to the standard RYGBP technique used in humans to achieve satisfactory results. Caloric intake decreased in some but not all animals, and was linked to feeding regimen. By postoperative day 30, animals weighed 5.7-29.1% less than their projected, non-operative weight. Serum assays of ghrelin and PYY were conducted, with results consistent with the procedure. CONCLUSIONS: The use of swine as a model for bariatric surgery has promise, but also has associated pitfalls that must be addressed for this to be an effective model.
BACKGROUND: Rodents have been used to examine physiologic changes after bariatric surgery, but differences in gastric/vagal anatomy may limit their utility. Swine may be a more appropriate animal model because of anatomic and physiologic similarities to humans. The aim of this study was to establish a survival model of Roux-en-Y gastric bypass (RYGBP) in swine and to evaluate its potential in studies of physiology. METHODS: 13 miniature swine, 5 Yucatan [26.4 +/- 1.6 kg], 4 Hanford [28.3 +/- 0.6 kg] and 4 other breed [54.9 +/- 6.2 kg] underwent open RYGBP, and were kept alive to 30 (n=4), 60 (n=1) or 90 (n=2) postoperative days. RESULTS: 4 early animals had staple-line leakage within 7 days from surgery and 1 animal experienced unmanageable pain at 42 days after surgery. One animal experienced immediate cardiopulmonary collapse. 58% of animals survived to their projected endpoint. Necropsy of 1 animal at its 90-day endpoint revealed a gastro-gastric fistula. Anatomic features in swine that differ from humans, such as thick perigastric membranes, required adjustment to the standard RYGBP technique used in humans to achieve satisfactory results. Caloric intake decreased in some but not all animals, and was linked to feeding regimen. By postoperative day 30, animals weighed 5.7-29.1% less than their projected, non-operative weight. Serum assays of ghrelin and PYY were conducted, with results consistent with the procedure. CONCLUSIONS: The use of swine as a model for bariatric surgery has promise, but also has associated pitfalls that must be addressed for this to be an effective model.
Authors: Francesco Rubino; Antonello Forgione; David E Cummings; Michel Vix; Donatella Gnuli; Geltrude Mingrone; Marco Castagneto; Jacques Marescaux Journal: Ann Surg Date: 2006-11 Impact factor: 12.969
Authors: David Nocca; Michel Gagner; Federico Cuenca Abente; Gian Mattia Del Genio; Kazuki Ueda; Ahmad Assalia; Thomas Rogula; Diego Bertani Journal: Obes Surg Date: 2005-04 Impact factor: 4.129
Authors: Francesco Rubino; Michel Gagner; Paolo Gentileschi; Subhash Kini; Shoji Fukuyama; John Feng; Ed Diamond Journal: Ann Surg Date: 2004-08 Impact factor: 12.969
Authors: Carel W le Roux; Simon J B Aylwin; Rachel L Batterham; Cynthia M Borg; Frances Coyle; Vyas Prasad; Sandra Shurey; Mohammad A Ghatei; Ameet G Patel; Stephen R Bloom Journal: Ann Surg Date: 2006-01 Impact factor: 12.969
Authors: Zachary P Neeb; Jason M Edwards; Mouhamad Alloosh; Xin Long; Eric A Mokelke; Michael Sturek Journal: Comp Med Date: 2010-08 Impact factor: 0.982
Authors: V Spinelli; F Lalloyer; G Baud; E Osto; M Kouach; M Daoudi; E Vallez; V Raverdy; J-F Goossens; A Descat; P Doytcheva; T Hubert; T A Lutz; S Lestavel; B Staels; F Pattou; A Tailleux Journal: Int J Obes (Lond) Date: 2016-03-22 Impact factor: 5.095
Authors: Malene M Birck; Andreas Vegge; Mikael Støckel; Ismail Gögenur; Thomas Thymann; Karsten P Hammelev; Per T Sangild; Axel K Hansen; Kirsten Raun; Pia von Voss; Thomas Eriksen Journal: Am J Transl Res Date: 2013-09-25 Impact factor: 4.060
Authors: Jonathan G Sham; Vlad V Simianu; Andrew S Wright; Skye D Stewart; Mouhamad Alloosh; Michael Sturek; David E Cummings; David R Flum Journal: J Diabetes Res Date: 2014-08-24 Impact factor: 4.011
Authors: Vlad V Simianu; Jonathan G Sham; Andrew S Wright; Skye D Stewart; Mouhamad Alloosh; Michael Sturek; David E Cummings; David R Flum Journal: Surg Sci Date: 2015-07-24