Literature DB >> 18097095

Overexpression, purification and crystallization of the tetrameric form of SorC sorbitol operon regulator.

Daniele de Sanctis1, Ana T Rêgo, David Marçal, Colin E McVey, Maria A Carrondo, Francisco J Enguita.   

Abstract

The sorbitol operon regulator (SorC) regulates the metabolism of L-sorbose in Klebsiella pneumonia. SorC was overexpressed in Escherichia coli and purified, and crystals were obtained of a tetrameric form. A single crystal showed X-ray diffraction to 3.20 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 91.6, b = 113.3, c = 184.1 A. Analysis of the molecular-replacement solution indicates the presence of four SorC molecules in the asymmetric unit.

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Year:  2007        PMID: 18097095      PMCID: PMC2373991          DOI: 10.1107/S1744309107060897

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN: 1744-3091


  12 in total

1.  The importance of alignment accuracy for molecular replacement.

Authors:  Robert Schwarzenbacher; Adam Godzik; Slawomir K Grzechnik; Lukasz Jaroszewski
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-06-22

2.  Positive and negative regulation of expression of the L-sorbose (sor) operon by SorC in Klebsiella pneumoniae.

Authors:  B M Wöhrl; U F Wehmeier; J W Lengeler
Journal:  Mol Gen Genet       Date:  1990-11

3.  Coot: model-building tools for molecular graphics.

Authors:  Paul Emsley; Kevin Cowtan
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-11-26

4.  Likelihood-enhanced fast translation functions.

Authors:  Airlie J McCoy; Ralf W Grosse-Kunstleve; Laurent C Storoni; Randy J Read
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-03-24

5.  Determination of protein molecular weight, hydration, and packing from crystal density.

Authors:  B W Matthews
Journal:  Methods Enzymol       Date:  1985       Impact factor: 1.600

6.  Purification and characterization of the DeoR repressor of Bacillus subtilis.

Authors:  X Zeng; H H Saxild; R L Switzer
Journal:  J Bacteriol       Date:  2000-04       Impact factor: 3.490

7.  L-Sorbose metabolism in Klebsiella pneumoniae and Sor+ derivatives of Escherichia coli K-12 and chemotaxis toward sorbose.

Authors:  G A Sprenger; J W Lengeler
Journal:  J Bacteriol       Date:  1984-01       Impact factor: 3.490

8.  Ligation independent cloning (LIC) as a rapid route to families of recombinant biocatalysts from sequenced prokaryotic genomes.

Authors:  Daniel Bonsor; Stephanie F Butz; Julianna Solomons; Stephanie Grant; Ian J S Fairlamb; Mark J Fogg; Gideon Grogan
Journal:  Org Biomol Chem       Date:  2006-01-30       Impact factor: 3.876

Review 9.  The integration of macromolecular diffraction data.

Authors:  Andrew G W Leslie
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-12-14

10.  Maximum-likelihood density modification.

Authors:  T C Terwilliger
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2000-08
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