OBJECTIVE: To examine the protective role of ischaemic preconditioning (IPC) in rat hearts using Tc-glucarate (GLA) and a stationary SPECT imager, FastSPECT. METHODS: Twenty-four rats with 30 min myocardial ischaemia and 150 min reperfusion (IR) were studied as follows. The IPC group (n=6) underwent IPC (five cycles of 4 min ligation of the left coronary artery and reflow) before IR. The control group (n=7) was treated by IR without IPC. The SPT group (n=6) was subjected to IPC and an adenosine antagonist, 8-(p-sulfophenyl)-theophylline (SPT). The vehicle group (n=5) received IPC and SPT carrier vehicle. GLA was delivered intravenously 30 min post-reperfusion, and 2-h dynamic cardiac images were acquired by FastSPECT. RESULTS: GLA showed 'hot-spot' accumulation in the ischaemic area-at-risk (IAR) and exhibited lower retention (% 5 min peak) in the IPC and vehicle groups (33.8+/-2.6 vs. 35.7+/-9.2, P>0.05) than in the control and SPT groups (63.1+/-5.3 vs. 54.8+/-4.8, P>0.05). The infarct size (% IAR) was larger in the control and SPT groups (48.2+/-6.3 vs. 41.7+/-6.3, P>0.05) than that in the IPC and vehicle groups (21.0+/-1.9 vs. 19.1+/-4.6, P>0.05). In terms of the ex-vivo IAR-to-normal radioactivity ratio, there was a statistical difference between the control and IPC groups (7.4+/-0.9 vs. 3.0+/-0.4), as well as the SPT and vehicle groups (7.4+/-1.0 vs. 3.4+/-0.5). CONCLUSION: IPC offers cardioprotection and relates to the activation of adenosine receptors in rat hearts. FastSPECT GLA imaging is not only useful in detecting early ischaemia-reperfusion injury, but also valuable in evaluating cardioprotection.
OBJECTIVE: To examine the protective role of ischaemic preconditioning (IPC) in rat hearts using Tc-glucarate (GLA) and a stationary SPECT imager, FastSPECT. METHODS: Twenty-four rats with 30 min myocardial ischaemia and 150 min reperfusion (IR) were studied as follows. The IPC group (n=6) underwent IPC (five cycles of 4 min ligation of the left coronary artery and reflow) before IR. The control group (n=7) was treated by IR without IPC. The SPT group (n=6) was subjected to IPC and an adenosine antagonist, 8-(p-sulfophenyl)-theophylline (SPT). The vehicle group (n=5) received IPC and SPT carrier vehicle. GLA was delivered intravenously 30 min post-reperfusion, and 2-h dynamic cardiac images were acquired by FastSPECT. RESULTS:GLA showed 'hot-spot' accumulation in the ischaemic area-at-risk (IAR) and exhibited lower retention (% 5 min peak) in the IPC and vehicle groups (33.8+/-2.6 vs. 35.7+/-9.2, P>0.05) than in the control and SPT groups (63.1+/-5.3 vs. 54.8+/-4.8, P>0.05). The infarct size (% IAR) was larger in the control and SPT groups (48.2+/-6.3 vs. 41.7+/-6.3, P>0.05) than that in the IPC and vehicle groups (21.0+/-1.9 vs. 19.1+/-4.6, P>0.05). In terms of the ex-vivo IAR-to-normal radioactivity ratio, there was a statistical difference between the control and IPC groups (7.4+/-0.9 vs. 3.0+/-0.4), as well as the SPT and vehicle groups (7.4+/-1.0 vs. 3.4+/-0.5). CONCLUSION:IPC offers cardioprotection and relates to the activation of adenosine receptors in rat hearts. FastSPECT GLA imaging is not only useful in detecting early ischaemia-reperfusion injury, but also valuable in evaluating cardioprotection.
Authors: R K Rowe; J N Aarsvold; H H Barrett; J C Chen; W P Klein; B A Moore; I W Pang; D D Patton; T A White Journal: J Nucl Med Date: 1993-03 Impact factor: 10.057
Authors: David R Okada; Gerald Johnson; Zhonglin Liu; Sonia D Hocherman; Ban-An Khaw; Robert D Okada Journal: J Nucl Med Date: 2004-04 Impact factor: 10.057
Authors: Zhonglin Liu; Harrison H Barrett; Gail D Stevenson; George A Kastis; Michael Bettan; Lars R Furenlid; Donald W Wilson; Koon Yan Pak Journal: J Nucl Med Date: 2004-07 Impact factor: 10.057