| Literature DB >> 18094112 |
Lidice Bernardo1, Alienys Izquierdo, Irina Prado, Delfina Rosario, Mayling Alvarez, Emidalys Santana, Jorge Castro, Rafael Martínez, Rosmari Rodríguez, Luis Morier, Gerardo Guillén, María G Guzmán.
Abstract
The goal of this study was to compare the immune response and the protection capacity induced by the dengue virus 2 (DENV-2) American and Asian genotypes in Macaca fascicularis monkeys. Animals were infected with American or Asian DENV-2 strains and challenged 1 year later with a DENV-2 Asian genotype strain. The viremia and monkey antibody levels were similar for the different strains after primary and secondary infection; however, the functionality of the antibody response was different. A limited viral replication was demonstrated after the secondary infection in all the monkeys. No virus was isolated in tissue culture, while reverse transcription-PCR showed a late positive reaction in four of five challenged monkeys. The immunoglobulin M response pattern and the detection of antibodies to specific proteins by Western blotting supported the protection data. Despite the demonstration of the protective effect after homologous challenge, a strong anamnestic antibody response was observed.Entities:
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Year: 2007 PMID： 18094112 PMCID： PMC2268260 DOI： 10.1128/CVI.00208-07
Source DB: PubMed Journal: Clin Vaccine Immunol ISSN： 1556-679X