Literature DB >> 18080841

Expression and purification of Zantedeschia aethiopica agglutinin in Escherichia coli.

Ling Lin1, Xue-Fen Liu, Ling-Chuan Hu, Yin Zhou, Xiao-Fen Sun, Ke-Xuan Tang.   

Abstract

Recombinant Zantedeschia aethiopica agglutinin (ZAA) was expressed in Escherichia coli as N-terminal His-tagged fusion. After induction with isopropylthio-beta-D-galactoside (IPTG), the recombinant ZAA was purified by metal-affinity chromatography. The purified ZAA protein was applied in anti-fungal assay and the result showed that recombinant ZAA had anti-fungal activity towards leaf mold (Fulvia fulva), one of the most serious phytopathogenic fungi causing significant yield loss of crops. This study suggests that ZAA could be an effective candidate in genetic engineering of plants for the control of leaf mold.

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Year:  2007        PMID： 18080841     DOI： 10.1007/s11033-007-9198-8

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  7 in total

1.  Expression and purification of Arisaema heterophyllum agglutinin in Escherichia coli.

Authors:  Xiuyun Zhao; Zhonghai Chen; Juan Lin; Weiwen Kong; Xiaofen Sun; Kexuan Tang
Journal:  J Plant Physiol       Date:  2005-06-14       Impact factor: 3.549

2.  Cloning and expression of a novel cDNA encoding a mannose-binding lectin from Dendrobium officinale.

Authors:  Zhonghai Chen; Xiaofen Sun; Kexuan Tang
Journal:  Toxicon       Date:  2005-03-15       Impact factor: 3.033

3.  Genomic cloning and characterization of a novel lectin gene from Zantedeschia aethiopica.

Authors:  Zhonghai Chen; Xiaofen Sun; Kexuan Tang
Journal:  Biosci Rep       Date:  2004-06       Impact factor: 3.840

4.  A chitin-binding lectin from stinging nettle rhizomes with antifungal properties.

Authors:  W F Broekaert; J VAN Parijs; F Leyns; H Joos; W J Peumans
Journal:  Science       Date:  1989-09-08       Impact factor: 47.728

5.  Molecular cloning and characterization of a novel mannose-binding lectin cDNA from Zantedeschia aethiopica.

Authors:  Zhonghai Chen; Yongzhen Pang; Xiaojun Liu; Xinglong Wang; Zhongxiang Deng; Xiaofen Sun; Kexuan Tang
Journal:  Biocell       Date:  2005-08       Impact factor: 1.254

6.  A new method for predicting signal sequence cleavage sites.

Authors:  G von Heijne
Journal:  Nucleic Acids Res       Date:  1986-06-11       Impact factor: 16.971

7.  Gastrodia anti-fungal protein from the orchid Gastrodia elata confers disease resistance to root pathogens in transgenic tobacco.

Authors:  K D Cox; D R Layne; R Scorza; G Schnabel
Journal:  Planta       Date:  2006-07-21       Impact factor: 4.116
  7 in total
  1 in total

1.  Shedding some light over the floral metabolism by arum lily (Zantedeschia aethiopica) spathe de novo transcriptome assembly.

Authors:  Elizabete de Souza Cândido; Gabriel da Rocha Fernandes; Sérgio Amorim de Alencar; Marlon Henrique e Silva Cardoso; Stella Maris de Freitas Lima; Vívian de Jesus Miranda; William Farias Porto; Diego Oliveira Nolasco; Nelson Gomes de Oliveira-Júnior; Aulus Estevão Anjos de Deus Barbosa; Robert Edward Pogue; Taia Maria Berto Rezende; Simoni Campos Dias; Octávio Luiz Franco
Journal:  PLoS One       Date:  2014-03-10       Impact factor: 3.240
  1 in total

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