Literature DB >> 16399011

Expression and purification of Arisaema heterophyllum agglutinin in Escherichia coli.

Xiuyun Zhao1, Zhonghai Chen, Juan Lin, Weiwen Kong, Xiaofen Sun, Kexuan Tang.   

Abstract

Recombinant Arisaema heterophyllum agglutinin (AHA) was expressed in Escherichia coli as N-terminal His-tagged fusions. After induction with isopropylthio-beta-D-galactoside, the recombinant AHA was purified by metal-affinity chromatography. The purified AHA protein was incorporated into artificial diet at 0.1% (w/v) concentration in insect bioassay trial and the result showed that artificial diet containing AHA could significantly inhibit the growth of the third-instar nymphs of peach potato aphid (Myzus persicae). This study suggested that AHA could be an effective candidate for the control of peach potato aphid, one of the most serious sap-sucking insect pests causing significant yield loss of crops.

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Year:  2005        PMID: 16399011     DOI: 10.1016/j.jplph.2005.01.006

Source DB:  PubMed          Journal:  J Plant Physiol        ISSN: 0176-1617            Impact factor:   3.549


  4 in total

1.  Molecular cloning and characterization of a mannose-binding lectin gene from Pinellia cordata.

Authors:  Ling Lin; Jie Lu; Hainian Zeng; Zhifeng Liang; Yin Zhou; Juan Lin; Xiaofen Sun; Kexuan Tang
Journal:  Mol Biol Rep       Date:  2007-10-12       Impact factor: 2.316

2.  Phylogenetic and specificity studies of two-domain GNA-related lectins: generation of multispecificity through domain duplication and divergent evolution.

Authors:  Els J M Van Damme; Sachiko Nakamura-Tsuruta; David F Smith; Maté Ongenaert; Harry C Winter; Pierre Rougé; Irwin J Goldstein; Hanqing Mo; Junko Kominami; Raphaël Culerrier; Annick Barre; Jun Hirabayashi; Willy J Peumans
Journal:  Biochem J       Date:  2007-05-15       Impact factor: 3.857

3.  Expression and purification of Zantedeschia aethiopica agglutinin in Escherichia coli.

Authors:  Ling Lin; Xue-Fen Liu; Ling-Chuan Hu; Yin Zhou; Xiao-Fen Sun; Ke-Xuan Tang
Journal:  Mol Biol Rep       Date:  2007-12-16       Impact factor: 2.316

4.  De novo sequencing and transcriptome assembly of Arisaema heterophyllum Blume and identification of genes involved in isoflavonoid biosynthesis.

Authors:  Chenkai Wang; Jinhang Zhu; Miaomiao Liu; Qingshan Yang; Jiawen Wu; Zegeng Li
Journal:  Sci Rep       Date:  2018-12-05       Impact factor: 4.379

  4 in total

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