| Literature DB >> 18080466 |
C Shu-hui Tan1, Cor W van Ingen, Joost A Stalpers.
Abstract
Lyophilization, the removal of water by freezing and then volatilization at low pressure and temperature, has been employed as a standard long-term preservation method for many filamentous fungi. The method outlined involves the use of standard shelf freeze-drying and skimmed milk as a suspending solution/lyoprotectant. This approach has been employed to freeze-dry the majority of the 50,000 fungal strains that have been successfully lyophilized at the Centraal bureau voor Schimmelcultures (CBS) culture collection (http://www.cbs.knaw.nl/).Entities:
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Year: 2007 PMID： 18080466 DOI： 10.1007/978-1-59745-362-2_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745