| Literature DB >> 18079727 |
Sean A McKenna1, Insil Kim, Elisabetta Viani Puglisi, Darrin A Lindhout, Colin Echeverría Aitken, R Andrew Marshall, Joseph D Puglisi.
Abstract
RNA synthesis using in vitro transcription by phage T7 RNA polymerase allows preparation of milligram quantities of RNA for biochemical, biophysical and structural investigations. Previous purification approaches relied on gel electrophoretic or gravity-flow chromatography methods. We present here a protocol for the in vitro transcription of RNAs and subsequent purification using fast-performance liquid chromatography. This protocol greatly facilitates production of RNA in a single day from transcription to purification.Entities:
Mesh：
Substances：
Year: 2007 PMID： 18079727 DOI： 10.1038/nprot.2007.480
Source DB: PubMed Journal: Nat Protoc ISSN： 1750-2799 Impact factor: 13.491