Literature DB >> 18078799

A method for analyzing lipid-modified proteins with mass spectrometry.

Tetsuro Ujihara1, Isamu Sakurai, Naoki Mizusawa, Hajime Wada.   

Abstract

In protein analysis using mass spectrometry, proteins are usually separated by electrophoresis and digested within the gel with proteases such as trypsin. However, analysis of lipid-modified proteins is difficult due to the low recovery of lipid-modified peptide fragments from the gel as well as their low ionization efficiency during mass spectrometry. In this study, we developed a simple extraction method with n-dodecyl-beta-D-maltoside following chloroform/methanol extraction that efficiently elutes lipid-modified fragments from gels. This method allowed us to analyze the structure of lipid-modified fragments, suggesting the applicability of the method for analysis of lipid-modified fragments by mass spectrometry.

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Year:  2007        PMID： 18078799     DOI： 10.1016/j.ab.2007.11.014

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

Review 1.  Proteomic approaches in research of cyanobacterial photosynthesis.

Authors:  Natalia Battchikova; Martina Angeleri; Eva-Mari Aro
Journal:  Photosynth Res       Date:  2014-10-31       Impact factor: 3.573

2.  MS-based cross-linking analysis reveals the location of the PsbQ protein in cyanobacterial photosystem II.

Authors:  Haijun Liu; Hao Zhang; Daniel A Weisz; Ilan Vidavsky; Michael L Gross; Himadri B Pakrasi
Journal:  Proc Natl Acad Sci U S A       Date:  2014-02-18       Impact factor: 11.205

3.  Identification of apolipoprotein N-acyltransferase (Lnt) in mycobacteria.

Authors:  Andreas Tschumi; Corrado Nai; Yolanda Auchli; Peter Hunziker; Peter Gehrig; Peter Keller; Thomas Grau; Peter Sander
Journal:  J Biol Chem       Date:  2009-08-06       Impact factor: 5.157

4.  The ppm operon is essential for acylation and glycosylation of lipoproteins in Corynebacterium glutamicum.

Authors:  Niloofar Mohiman; Manuela Argentini; Sarah M Batt; David Cornu; Muriel Masi; Lothar Eggeling; Gurdyal Besra; Nicolas Bayan
Journal:  PLoS One       Date:  2012-09-28       Impact factor: 3.240

Review 5.  The Use of Advanced Mass Spectrometry to Dissect the Life-Cycle of Photosystem II.

Authors:  Daniel A Weisz; Michael L Gross; Himadri B Pakrasi
Journal:  Front Plant Sci       Date:  2016-05-10       Impact factor: 5.753

6.  Lipoprotein Glycosylation by Protein-O-Mannosyltransferase (MAB_1122c) Contributes to Low Cell Envelope Permeability and Antibiotic Resistance of Mycobacterium abscessus.

Authors:  Katja Becker; Klara Haldimann; Petra Selchow; Lukas M Reinau; Michael Dal Molin; Peter Sander
Journal:  Front Microbiol       Date:  2017-11-02       Impact factor: 5.640

7.  Mass spectrometry analysis of the photosystem II assembly factor Psb27 revealed variations in its lipid modification.

Authors:  Jan Lambertz; Pasqual Liauw; Julian P Whitelegge; Marc M Nowaczyk
Journal:  Photosynth Res       Date:  2021-12-15       Impact factor: 3.429

8.  Crystal structure of CyanoQ from the thermophilic cyanobacterium Thermosynechococcus elongatus and detection in isolated photosystem II complexes.

Authors:  Franck Michoux; Marko Boehm; Wojciech Bialek; Kenji Takasaka; Karim Maghlaoui; James Barber; James W Murray; Peter J Nixon
Journal:  Photosynth Res       Date:  2014-05-18       Impact factor: 3.573

9.  Lipoproteins of slow-growing Mycobacteria carry three fatty acids and are N-acylated by apolipoprotein N-acyltransferase BCG_2070c.

Authors:  Juliane K Brülle; Andreas Tschumi; Peter Sander
Journal:  BMC Microbiol       Date:  2013-10-05       Impact factor: 3.605

10.  N-Terminal Lipid Modification Is Required for the Stable Accumulation of CyanoQ in Synechocystis sp. PCC 6803.

Authors:  Andrea D Juneau; Laurie K Frankel; Terry M Bricker; Johnna L Roose
Journal:  PLoS One       Date:  2016-09-22       Impact factor: 3.240
  10 in total

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