| Literature DB >> 18077095 |
Chul-min Kim1, Lidia Beatriz Conza Blanco, Andy Alhassan, Hiroshi Iseki, Naoaki Yokoyama, Xuenan Xuan, Ikuo Igarashi.
Abstract
We developed a TaqMan real-time polymerase chain reaction (PCR) assay for the quantitative detection of Theileria equi from the in vitro-cultured parasite and field blood samples collected from horses living in Ghana and Brazil. The detection limit for the assay was determined to be 1.5 parasites/microl per sample, and the quantitative capacity was demonstrated using the in vitro-cultured parasite. For field applications, the real-time PCR assay was compared to a previously established nested PCR assay used as the gold standard for the real-time PCR assay. Of 65 field blood samples, 46 samples were T. equi-positive in the nested PCR assay, while the real-time PCR assay also detected the parasite in all 46 of the nested PCR-positive samples but did not detect T. equi in the remaining 19 negative blood samples. This quantitative real-time PCR assay provides a valuable tool for fast laboratory diagnostic assessment of T. equi infection in horses.Entities:
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Year: 2007 PMID： 18077095 DOI： 10.1016/j.vetpar.2007.10.023
Source DB: PubMed Journal: Vet Parasitol ISSN： 0304-4017 Impact factor: 2.738