[Separation and determination of oleanolic acid and ursolic acid from Cornus officinalis by capillary electrophoresis].

A cycledextrin-modified micellar electrokinetic chromatography technique for separating and determination oleanolic acid and ursolic acid in the fruit of Cornus officinalis was developed. The two bio-active components was completely separated using a buffer containing 60 mmol/L SDS, 2.5 mmol/L NaH2PO4, 2.5 mmol/L Na2B4O7, 8 mmol/L DM beta-CD,8 mmol/L beta-CD and 30% (v/v) 2-propanol. The correlation cofficients of the linear calibration graphs for oleanolic acid and ursolic acid are 0. 9996 and 0.9995, respectively. The effects of SDS, 2-propanol, dimethyl-beta-cyclodextrin, beta-cyclodextrin, NaH2PO4, Na2B4O7 and pH on separation were investigated.