Literature DB >> 18059867

Membrane imaging by simultaneous second-harmonic generation and two-photon microscopy.

L Moreaux, O Sandre, M Blanchard-Desce, J Mertz.   

Abstract

We demonstrate that simultaneous second-harmonic generation (SHG) and two-photon-excited fluorescence (TPEF) can be used to rapidly image biological membranes labeled with a styryl dye. The SHG power is made compatible with the TPEF power by use of near-resonance excitation, in accord with a model based on the theory of phased-array antennas, which shows that the SHG radiation is highly structured. Because of its sensitivity to local asymmetry, SHG microscopy promises to be a powerful tool for the study of membrane dynamics.

Entities:  

Year:  2000        PMID： 18059867     DOI： 10.1364/ol.25.000320

Source DB:  PubMed          Journal:  Opt Lett        ISSN： 0146-9592            Impact factor:   3.776


  24 in total

1.  Sensitivity of second harmonic generation from styryl dyes to transmembrane potential.

Authors:  Andrew C Millard; Lei Jin; Mei-De Wei; Joseph P Wuskell; Aaron Lewis; Leslie M Loew
Journal:  Biophys J       Date:  2004-02       Impact factor: 4.033

2.  Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence.

Authors:  Aikaterini Zoumi; Alvin Yeh; Bruce J Tromberg
Journal:  Proc Natl Acad Sci U S A       Date:  2002-08-12       Impact factor: 11.205

3.  Studies of chi(2)/chi(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy.

Authors:  Shi-Wei Chu; Szu-Yu Chen; Gia-Wei Chern; Tsung-Han Tsai; Yung-Chih Chen; Bai-Ling Lin; Chi-Kuang Sun
Journal:  Biophys J       Date:  2004-06       Impact factor: 4.033

4.  Imaging coronary artery microstructure using second-harmonic and two-photon fluorescence microscopy.

Authors:  Aikaterini Zoumi; Xiao Lu; Ghassan S Kassab; Bruce J Tromberg
Journal:  Biophys J       Date:  2004-10       Impact factor: 4.033

Review 5.  Molecular contrast optical coherence tomography: a review.

Authors:  Changhuei Yang
Journal:  Photochem Photobiol       Date:  2005 Mar-Apr       Impact factor: 3.421

6.  Intraocular multiphoton microscopy with subcellular spatial resolution by infrared femtosecond lasers.

Authors:  Bao-Gui Wang; Karsten Koenig; Iris Riemann; Reimar Krieg; Karl-Juergen Halbhuber
Journal:  Histochem Cell Biol       Date:  2006-05-04       Impact factor: 4.304

7.  Second-harmonic generation imaging of membrane potential with photon counting.

Authors:  Jiang Jiang; Rafael Yuste
Journal:  Microsc Microanal       Date:  2008-12       Impact factor: 4.127

8.  Photon counting, censor corrections, and lifetime imaging for improved detection in two-photon microscopy.

Authors:  Jonathan D Driscoll; Andy Y Shih; Satish Iyengar; Jeffrey J Field; G Allen White; Jeffrey A Squier; Gert Cauwenberghs; David Kleinfeld
Journal:  J Neurophysiol       Date:  2011-04-06       Impact factor: 2.714

9.  In vivo imaging of myelin in the vertebrate central nervous system using third harmonic generation microscopy.

Authors:  Matthew J Farrar; Frank W Wise; Joseph R Fetcho; Chris B Schaffer
Journal:  Biophys J       Date:  2011-03-02       Impact factor: 4.033

10.  Thermal transitions of fibrillar collagen unveiled by second-harmonic generation microscopy of corneal stroma.

Authors:  Paolo Matteini; Riccardo Cicchi; Fulvio Ratto; Dimitrios Kapsokalyvas; Francesca Rossi; Marella de Angelis; Francesco S Pavone; Roberto Pini
Journal:  Biophys J       Date:  2012-09-19       Impact factor: 4.033
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