Literature DB >> 180528

Affinity label for beta-adrenergic receptor in turkey erythrocytes.

D Atlas, M L Steer, A Levitzki.   

Abstract

The compound N-[2-hydroxy-3-(1-naphthoxy)-propyl]-N'-bromoacetylethylenediamine (NHNP-NBE) was found to label covalently the beta-adrenergic receptor in turkey erythrocytes. The compound inhibits irreversibly 1-epinephrine-dependent adenylate cyclase activity [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] in the whole turkey erythrocyte as well as in the erythrocyte membranes possessing the beta-receptor. The affinity label blocks, also irreversibly, the specific [3H] propranolol binding, whereas other bromoacetyl compounds tested have no effect on binding, even at high concentrations, which cause enzyme inactivation. 1-Epinephrine and propranolol offer protection against the affinity label in whole turkey erythrocytes as well as in membranes prepared from these cells. The potential usefulness of an irreversible beta-antagonist is discussed.

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Year:  1976        PMID： 180528      PMCID： PMC430419          DOI： 10.1073/pnas.73.6.1921

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  16 in total

1.  Ligand specificity and characteristics of the beta-adrenergic receptor in turkey erythrocyte plasma membranes.

Authors:  A Levitzki; N Sevilia; D Atlas; M L Steer
Journal:  J Mol Biol       Date:  1975-09-05       Impact factor: 5.469

2.  The control of adenylate cyclase by calcium in turkey erythrocyte ghosts.

Authors:  M L Steer; A Levitzki
Journal:  J Biol Chem       Date:  1975-03-25       Impact factor: 5.157

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  The synthesis and local anesthetic properties of aryloxypropanolamines.

Authors:  H R ING; W E ORMEROD
Journal:  J Pharm Pharmacol       Date:  1952-01       Impact factor: 3.765

5.  Potential probe for isolation of the beta-adrenoceptor, chloropractolol.

Authors:  M Erez; M Weinstock; S Cohen; G Shtacher
Journal:  Nature       Date:  1975-06-19       Impact factor: 49.962

6.  Stereospecific (3H)(minus)-alprenolol binding sites, beta-adrenergic receptors and adenylate cyclase.

Authors:  R J Lefkowitz; C Mukherjee; M Coverstone; M G Caron
Journal:  Biochem Biophys Res Commun       Date:  1974-09-23       Impact factor: 3.575

7.  Activation of pigeon erythrocyte membrane adenylate cyclase by guanylnucleotide analogues and separation of a nucleotide binding protein.

Authors:  T Pfeuffer; E J Helmreich
Journal:  J Biol Chem       Date:  1975-02-10       Impact factor: 5.157

8.  Beta-adrenergic receptor: stereospecific interaction of iodinated beta-blocking agent with high affinity site.

Authors:  G D Aurbach; S A Fedak; C J Woodard; J S Palmer; D Hauser; F Troxler
Journal:  Science       Date:  1974-12-27       Impact factor: 47.728

9.  A protein binding assay for adenosine 3':5'-cyclic monophosphate.

Authors:  A G Gilman
Journal:  Proc Natl Acad Sci U S A       Date:  1970-09       Impact factor: 11.205

10.  Identification of cardiac beta-adrenergic receptors by (minus) [3H]alprenolol binding.

Authors:  R W Alexander; L T Williams; R J Lefkowitz
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205
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  6 in total

1.  Probing of beta-adrenergic receptors by novel fluorescent beta-adrenergic blockers.

Authors:  D Atlas; A Levitzki
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

2.  Photoaffinity label for the beta-adrenergic receptor: synthesis and effects on isoproterenol-stimulated adenylate cyclase.

Authors:  S M Wrenn; C J Homcy
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205

3.  Studies on agents with mixed NO-dependent vasodilating and beta-blocking activities.

Authors:  D Boschi; A Di Stilo; C Cena; M Lolli; R Fruttero; A Gasco
Journal:  Pharm Res       Date:  1997-12       Impact factor: 4.200

4.  Quantitative relationship between beta-adrenergic receptor number and physiologic responses as studied with a long-lasting beta-adrenergic antagonist.

Authors:  W L Terasaki; J Linden; G Brooker
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

5.  Irreversible blockade of beta-adrenergic receptors with a bromoacetyl derivative of pindolol.

Authors:  V Homburger; H Gozlan; R Bouhelal; M Lucas; J Bockaert
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1985-01       Impact factor: 3.000

6.  Association of turkey erythrocyte beta-adrenoceptors with a specific lipid component.

Authors:  A Bar-Sinai; Y Aldouby; M Chorev; A Levitzki
Journal:  EMBO J       Date:  1986-06       Impact factor: 11.598
  6 in total

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