Evidence for the role of zinc on the performance of dibenzothiophene desulfurization by Gordonia alkanivorans strain 1B.

Gordonia alkanivorans strain 1B is able to desulfurize dibenzothiophene (DBT) to 2-hydroxybiphenyl (2-HBP), the final product of the 4S pathway. However, both the cell growth and the rate of desulfurization can be largely affected by the nutrient composition of the growth medium due to cofactor requirements of many enzymes involved in the biochemical pathways. In this work, the effect of several metal ions on the growth and DBT desulfurization by G. alkanivorans was studied. From all the metal ions tested, only the absence of zinc significantly affected the cell growth and the desulfurization rate. By increasing the concentration of Zn from 1 to 10 mg L(-1), 2-HBP productivity was improved by 26%. The absence of Zn(2+), when sulfate was also used as the only sulfur source, did not cause any difference in the bacterial growth. Resting cells grown in the presence of Zn(2+) exhibited a 2-HBP specific productivity of 2.29 micromol g(-1) (DCW) h(-1), 7.6-fold higher than the specific productivity obtained by resting cells grown in the absence of Zn(2+) (0.30 micromol g(-1) (DCW) h(-1)). These data suggests that zinc might have a key physiological role in the metabolism of DBT desulfurization.