| Literature DB >> 18031967 |
Valentina Porta1, Simone Grigoleto Schramm, Eunice Kazue Kano, Eunice Emiko Koono, Yara Popst Armando, Kazuo Fukuda, Cristina Helena Dos Reis Serra.
Abstract
In this study, a simple, rapid and sensitive HPLC method with UV detection is described for determination of metformin in plasma samples from bioequivalence assays. Sample preparation was accomplished through protein precipitation with acetonitrile and chromatographic separation was performed on a reversed-phase phenyl column at 40 degrees C. Mobile phase consisted of a mixture of phosphate buffer and acetonitrile at flow rate of 1.0 ml/min. Wavelength was set at 236 nm. The method was applied to a bioequivalence study of two drug products containing metformin, and allowed determination of metformin at low concentrations with a higher throughput than previously described methods.Entities:
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Year: 2007 PMID： 18031967 DOI： 10.1016/j.jpba.2007.10.007
Source DB: PubMed Journal: J Pharm Biomed Anal ISSN： 0731-7085 Impact factor: 3.935