Literature DB >> 18028864

DNA digestion to deoxyribonucleoside: a simplified one-step procedure.

Eoin P Quinlivan1, Jesse F Gregory.   

Abstract

We present a simple and inexpensive 'one-step' protocol for the hydrolysis of DNA to deoxyribonucleosides. Unlike the older DNA hydrolysis protocol which is cumbersome and labor intensive, this new protocol is ideal for high-throughput assays and is suitable automation. Using this protocol we were able to hydrolyze several hundred samples within an 8-hour period. The new protocol is fully compatible with LC-MS/MS and gives similar recoveries for all five major deoxyribonucleosides when compared to the older protocol.

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Year:  2007        PMID： 18028864      PMCID： PMC2239294          DOI： 10.1016/j.ab.2007.09.031

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  12 in total

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