Literature DB >> 1802702

Culture of Borrelia burgdorferi on six solid media.

V Preac-Mursic1, B Wilske, S Reinhardt.   

Abstract

Six solid substrates were compared for their support of growth of ten isolates of Borrelia burgdorferi, the causative agent of Lyme borreliosis. The following substrates with or without rabbit serum and bovine serum albumin were tested: BSK agar, BHIAM agar, TAROM agar, MEM agar, MKP agar and PMR agar. After incubation in a candle jar and a GasPak for two to four weeks, Borrelia colonies were counted and characterized. Colony morphology was related more to the growth substrate than to the characteristics of the various Borrelia burgdorferi isolates. Culture on PMR agar resulted in the highest recovery rate and the best colony formation, with a size variation of 0.3-3.0 mm. Culture of Borrelia burgdorferi on solid media may facilitate the diagnosis of Lyme borreliosis by enabling easier biological and genetical analysis of isolates and more accurate differentiation of strains.

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Year:  1991        PMID： 1802702     DOI： 10.1007/BF01984935

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  10 in total

1.  Taxonomy of Borrelia spp.

Authors:  B Wilske; J F Anderson; G Baranton; A G Barbour; K Hovind-Hougen; R C Johnson; V Preac-Mursic
Journal:  Scand J Infect Dis Suppl       Date:  1991

2.  Cultivation of Borrelia hermsi.

Authors:  R Kelly
Journal:  Science       Date:  1971-07-30       Impact factor: 47.728

3.  Lyme disease-a tick-borne spirochetosis?

Authors:  W Burgdorfer; A G Barbour; S F Hayes; J L Benach; E Grunwaldt; J P Davis
Journal:  Science       Date:  1982-06-18       Impact factor: 47.728

4.  Clonal polymorphisms of outer membrane protein OspB of Borrelia burgdorferi.

Authors:  V G Bundoc; A G Barbour
Journal:  Infect Immun       Date:  1989-09       Impact factor: 3.441

5.  In vitro and in vivo susceptibility of Borrelia burgdorferi.

Authors:  V P Mursic; B Wilske; G Schierz; M Holmburger; E Süss
Journal:  Eur J Clin Microbiol       Date:  1987-08       Impact factor: 3.267

6.  Cultivation and characterization of spirochetes from cerebrospinal fluid of patients with Lyme borreliosis.

Authors:  M Karlsson; K Hovind-Hougen; B Svenungsson; G Stiernstedt
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

7.  Colony formation and morphology in Borrelia burgdorferi.

Authors:  T J Kurtti; U G Munderloh; R C Johnson; G G Ahlstrand
Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948

8.  European Borrelia burgdorferi isolated from humans and ticks culture conditions and antibiotic susceptibility.

Authors:  V Preac-Mursic; B Wilske; G Schierz
Journal:  Zentralbl Bakteriol Mikrobiol Hyg A       Date:  1986-12

9.  Immunochemical and immunological analysis of European Borrelia burgdorferi strains.

Authors:  B Wilske; V Preac-Mursic; G Schierz; K V Busch
Journal:  Zentralbl Bakteriol Mikrobiol Hyg A       Date:  1986-12

10.  Isolation and cultivation of Lyme disease spirochetes.

Authors:  A G Barbour
Journal:  Yale J Biol Med       Date:  1984 Jul-Aug
  10 in total
  12 in total

1.  Improvement of Lyme borreliosis serodiagnosis by a newly developed recombinant immunoglobulin G (IgG) and IgM line immunoblot assay and addition of VlsE and DbpA homologues.

Authors:  Gereon Goettner; Ulrike Schulte-Spechtel; Ruth Hillermann; Gabi Liegl; Bettina Wilske; Volker Fingerle
Journal:  J Clin Microbiol       Date:  2005-08       Impact factor: 5.948

2.  Dynamics of dissemination and outer surface protein expression of different European Borrelia burgdorferi sensu lato strains in artificially infected Ixodes ricinus nymphs.

Authors:  Volker Fingerle; Sandra Rauser; Bettina Hammer; Olaf Kahl; Christiane Heimerl; Ulrike Schulte-Spechtel; Lise Gern; Bettina Wilske
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

Review 3.  Diagnosis of lyme borreliosis.

Authors:  Maria E Aguero-Rosenfeld; Guiqing Wang; Ira Schwartz; Gary P Wormser
Journal:  Clin Microbiol Rev       Date:  2005-07       Impact factor: 26.132

4.  Diversity of OspA and OspC among cerebrospinal fluid isolates of Borrelia burgdorferi sensu lato from patients with neuroborreliosis in Germany.

Authors:  B Wilske; U Busch; H Eiffert; V Fingerle; H W Pfister; D Rössler; V Preac-Mursic
Journal:  Med Microbiol Immunol       Date:  1996-02       Impact factor: 3.402

5.  Formation and cultivation of Borrelia burgdorferi spheroplast-L-form variants.

Authors:  V P Mursic; G Wanner; S Reinhardt; B Wilske; U Busch; W Marget
Journal:  Infection       Date:  1996 May-Jun       Impact factor: 3.553

6.  Immunoblot using recombinant antigens derived from different genospecies of Borrelia burgdorferi sensu lato.

Authors:  B Wilske; V Fingerle; V Preac-Mursic; S Jauris-Heipke; A Hofmann; H Loy; H W Pfister; D Rössler; E Soutschek
Journal:  Med Microbiol Immunol       Date:  1994-02       Impact factor: 3.402

7.  Immunological and molecular polymorphisms of OspC, an immunodominant major outer surface protein of Borrelia burgdorferi.

Authors:  B Wilske; V Preac-Mursic; S Jauris; A Hofmann; I Pradel; E Soutschek; E Schwab; G Will; G Wanner
Journal:  Infect Immun       Date:  1993-05       Impact factor: 3.441

8.  Recovery of Borrelia burgdorferi by filtration.

Authors:  D A Jobe; S M Callister; R F Schell
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948

9.  Active immunization with pC protein of Borrelia burgdorferi protects gerbils against B. burgdorferi infection.

Authors:  V Preac-Mursic; B Wilske; E Patsouris; S Jauris; G Will; E Soutschek; S Rainhardt; G Lehnert; U Klockmann; P Mehraein
Journal:  Infection       Date:  1992 Nov-Dec       Impact factor: 3.553

10.  Comparative analysis of the Borrelia garinii genome.

Authors:  G Glöckner; R Lehmann; A Romualdi; S Pradella; U Schulte-Spechtel; M Schilhabel; B Wilske; J Sühnel; M Platzer
Journal:  Nucleic Acids Res       Date:  2004-11-16       Impact factor: 16.971
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