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Literature DB >> 18024676

Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure.

Leen Baert¹, Christiane E Wobus, Els Van Coillie, Larissa B Thackray, Johan Debevere, Mieke Uyttendaele.

Abstract

The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

Entities: Species

Mesh：

Substances：
RNA, Viral

Year: 2007 PMID： 18024676 PMCID： PMC2223245 DOI： 10.1128/AEM.01039-07

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

23 in total

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9. Norwalk virus RNA is infectious in mammalian cells.

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Review 10. Foodborne viruses: an emerging problem.

Authors: Marion Koopmans; Erwin Duizer
Journal: Int J Food Microbiol Date: 2004-01-01 Impact factor: 5.277

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5. Inactivation of murine norovirus 1 and Bacteroides fragilis phage B40-8 by mesophilic and thermophilic anaerobic digestion of pig slurry.

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6. Quantitative PCR for determining the infectivity of bacteriophage MS2 upon inactivation by heat, UV-B radiation, and singlet oxygen: advantages and limitations of an enzymatic treatment to reduce false-positive results.

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