3-Formylchromones IV. The rearrangement of 3-formylchromone enamines as a simple, facile route to novel pyrazolo[3,4-b]pyridines and the synthetic utility of the latter.

One-pot and facile preparations of 6-(2-hydroxy-5-R-benzoyl)-4-methyl-2-R1- pyrazolo[3,4-b]pyridines 4a-o are described, using the reaction of 3-formyl chromones 1 with 5-amino-1-R1-pyrazoles 2. An enamine-intermediate 2-ethyloxy-6-R-3-(3-methyl-1- phenylpyrazol-5-ylaminomethylene)chroman-4-one 3 was isolated at lower temperatures. Acyloxy-derivatives 5 of compounds 4 were obtained by acylation with acid chlorides or acid anhydrides. Coumarins 6 substituted at the 3- and 4-positions were prepared from the pyrazolo[3,4-b]pyridines 4 by condensation reactions and hydrazones 7 were formed from their reaction with 2,4-dinitrophenyl hydrazine. Reactions under microwave irradiation proceeded significantly faster and with high yields.

Introduction

In our previous synthetic and theoretical studies [1,2] we reported on the reactions of 3-formyl chromones 1 with primary amine derivatives of benzothiazole and aromatic acids, respectively. These studies confirmed that the investigated amines undergo 1,2- or 1,4-additions to 3-formyl chromones 1, forming a chroman-4-one enamine system as a main product. The relevant theoretical and kinetic studies of the reaction pathways of some 2- and 3-formylchromones with amine derivatives were published in the preceding papers [3,4,5,6] of this series. In general, 3-formylchromones 1 readily react with primary amines in an alcoholic medium yielding an enamine-adduct which rarely reacts further to give the corresponding Schiff base [7,10,11]. The important role of 3-formylchromones 1 as versatile synthons in heterocyclic chemistry as well as their pharmaceutical importance is well known [8,9,11]. These compounds also have interesting photochemical properties [7].

Our investigations of influence of microwave radiation – a widely used way to selectively excite primarily the polar components of reactants and solvent – on 3-formylchromone reactions were also reported elsewhere [12,13]. The main goal of this work was to examine the reaction of 1-substituted 5- aminopyrazoles 2 with 3-formylchromones 1 using classical and microwave heating, respectively.

Results and Discussion

In this article we report on a very convenient and smooth, one-pot method for the preparation of substituted 3-(2-hydroxybenzoyl)pyrazolo[3,4-b]pyridines 4a-o. Our results demonstrate a straight-forward entry to these pyrazolo[3,4-b]pyridine derivatives, which contain synthetically useful hydroxyl and carbonyl groups, and are formed via opening of the γ-pyrone ring and subsequent rearrangement of intermediate I with electrophilic substitution on the pyrazole ring. The 3-formylchromone starting compounds 1 are easily available from phenols according to Nohara [14,15].

This synthesis of pyrazolo[3,4-b]pyridine derivatives 4a-o was found to be very simple. Products were obtained by heating to reflux an equimolar mixture of 3-formylchromones 1 and 5-amino-pyrazole (2) in ethanol, using p-toluenesulfonic acid as catalyst (Scheme 1). The duration of the reflux (2 to 4 hours) was found to be important for the purity of the final rearranged products. The progress of the reaction can readily be monitored visually. Products 4 are of a pale yellow to white color but the intermediates are bright yellow.

Scheme 1

The reaction kinetics depend on the substituents R, R1 and the solvent used. It was found that the methyl group (+I, +M effects) and similar substituents (H-) speeded up the formation of the final products. On the other hand, the compounds containing nitro groups on the chromone or pyrazole rings remained longer in the intermediate state (4 hours) before completing the reaction.

Ethanol was found to be more suitable for the synthesis of compounds 4 than other representative solvents like dioxane or toluene, as compared to these, ethanol facilitated faster reactions with higher yields and gave better product purities. This work as well as the previous kinetic studies [6] showed that alcohols are in general very good solvents for all reaction intermediates. This observation can be understood by noting that an alcohol, as a weak nucleophile, takes on a catalytic and stabilizing function after addition on to the chromanone ring and to the intermediates of this many-step-reaction, whereby forming complexes with lower energy.

In Scheme 1 a probable mechanism for the formation of compounds 4 is proposed. Our synthetic studies and kinetic measurements [6] showed that step ii – addition of aminopyrazole and alcohol to the aldehydes – is faster then the subsequent step iv which includes electrophilic attack of the opened chromanone system (intermediate I) at the C-12 position of pyrazole ring and rearrangement. The shorter reaction time resulted in a final product with a certain amount of impurities. The 1H-NMR-spectra and TLC showed that enamine-adducts or opened-intermediates I are the main impurities in the final products 4. We observed that prolonging the duration of the reflux in ethanol provided the products 4 with higher purity. These products 4 are stable enough and further heating in ethanol does not change the composition of the reaction mixtures.

The role of the enamine-adduct 3a as a reaction intermediate was confirmed by its isolation from the reaction mixtures at lower temperature (-10°C). Nitro derivative 3b show higher stability than the methyl analogue, and can still be isolated as the main product even after 20 minutes of reflux. Enamines, too, rearrange into the final products 4 after prolonged refluxing of the reaction mixtures.

Synthesis under microwave irradiation proceeded significantly faster. The reaction time dropped down to 6 to 25 minutes under exposure to microwaves at 800 W and produced clean products 4 in high yields (90%). However, no isolation of intermediate 3 was possible in this case.

The structures of the rearranged compounds were proven by elemental analysis and by 1H- and 13C-NMR spectra. The assignment of the 1H chemical shifts was deduced from the signal multiplicities and from the HH COSY spectra. The appropriate assignment of the 13C chemical shifts was based on the HMQC spectra for the C-H carbons and for the quaternary carbons from the coupled 13C spectrum (compound 4a). All of the NMR spectra indicate that the products 4 contain the pyridine ring. This conclusion is based on the observation of typical pyridine-ring shift values of 8.96 (H-2) and of 8.46 (H-4) respectively, and of their coupling constant J(2,4)=1.1 Hz (for compound 4a). The presence of the doublet-doublet signal in the 13C coupled spectrum at 151.5 δ (C-4a), with the coupling constants 3J(C, H-2) = 13.4 Hz and 3J(C, H-4) = 6.8 Hz confirms that the pyrazole ring is connected with the pyridine ring in the (3,4-b) position.

The detailed analysis of the 13C-NMR data of compound 4a (R = H), used as a reference compound, is presented in Table 1. The similar data for the compounds bearing other R substituents are available upon request from the corresponding author.

Table 1

Physical data of the compounds 4

No ------- Yield		Compound name
		Formula / MW		Melting point			Elemental analysis
4a-------89%		3-(2-hydroxybenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine
		C20H15N3O2 329.4		120 –121 °C			Calc.: 72.94 %C; 4.59 %H; 12.76 %N Found: 73.12 %C; 4.54 %H; 12.87 %N
		13C-NMR (CDCl3) δ(ppm), J(Hz): 12.73 q, J(C,H)=127.9(C-8); 116.57q, 3J(C,H)=2.9(C-4a);118.98dt, J(C,H)=162.3, 3J(C,H)=7.3 (C-12);119.23dd, J(C,H)=163.6, 3J(C,H)=9.2(C-14);119.59dt, 3 J(C,H)=4.7, 3J(C,H)=7.6(C-10);121.45dt, J(C,H)=161.6, 3J(C,H)=7.5(C-17,21);126.55dt, J(C,H)=162.9, 3J(C,H)=7.6(C-19);127.54d, 3J(C,H-4)=8.0(C-5); 129.41dd, J(C,H)=161.5, 3J(C,H) = 8.3(C-18,20);131.87dd, J(C,H)=166.1, 3J(C,H)=6.0(C-4);133.27dd, J(C,H)=160.2, 3J(C,H)=9.0(C-15);136.85dd, J(C,H)=159.8, 3J(C,H)=9.2(C-13);139.24t, 3 J(C,H)=8.0(C16);144.50qd, 2J(C,H)=7.1, 3J(C,H)=2.5(C-16);150.30dd, J(C,H)=183.3, 3J(C,H)=5.5(C-2);151.54dd, 3J(C,H-2)=13.4, 3J(C,H-4)=6.8(C-7a);163.43dd, 3J(C,H) 7.5,6.8 (C-11), 199.11t, 3J(C,H) = 4.4(C-9).
4b		3-(2-hydroxy-5-methylbenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine
		C21H17N3O2 343.4		142-144 °C		Calc.: 73.38 %C; 4.99 %H; 12.23%N Found: 73.22 %C; 4.84 %H; 12.27 %N
-------
92%
4c		3-(2-hydroxy-5-fluorobenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine
		C20H14FN3O2 339.4		155-156 °C		-----
-------
87%
4d		3-(2-hydroxy-5-bromobenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine
		C20H14 BrN3O2 408.4		162-163° C		Calc.: 58.82 %C; 3.43 %H; 10.21%N Found: 58.62 %C; 3.21 %H; 10.01 %N
-------
87%
4e		3-(2-hydroxy-5-chlorobenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine
		C20H14 ClN3O2 363.2		160-162 °C		Calc.: 65.97 %C; 3.84 %H; 11.54%N; 9.74 %Cl Found: 66.22 %C; 3.88 %H;11.34%N; 9.70%Cl
-------
89%
4f		3-(2-hydroxy-5-nitrobenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine
		C20H14N4O4 374.4		207-208 °C		Calc.: 77.36 %C; 4.52 %H; 18.07%N Found: 77.48 %C; 4.58 %H; 18.14%N
-------
70%
4g		3-(2-hydroxy-5-methylbenzoyl)-5-methyl-7-(4-nitrophenyl)pyrazolo[3,4-b] pyridine
		C21H16N4O4 388.4		224-226 °C		Calc.: 64.94 % C; 4.15 % H; 14.43 % N Found: 64.78 % C; 4.03 % H; 14.37 % N
-------
73%
4h		3-(2-hydroxy-5-fluorobenzoyl)-5-methyl-7-4-(nitrophenyl)pyrazolo[3,4-b] pyridine
		C20H13 FN4O4 392.4		222 -224 °C		----------
-------
87%
4i		3-(2-hydroxy-5-bromobenzoyl)-5-methyl-7-(4-nitrophenyl)pyrazolo[3,4-b] pyridine
		C20H13 BrN4O4 329.4		260-261 °C		Calc.: 53.00 % C; 2.89 % H; 12.36 % N; 17.63 %Br Found: 53.19% C; 2.68 % H; 12.11% N;17.42 %Br
-------
82%
4j		3-(2-hydroxy-5-chlorobenzoyl)-5-methyl-7-4-(nitrophenyl)pyrazolo[3,4-b] pyridine
		C20H13 ClN4O4 408.8		239-241 °C		Calc.: 58.76% C; 3.21% H; 13.71% N; 8.67%Cl Found: 58.67 %C; 3.11% H; 13.68% N; 8.58%Cl
-------
88%
4k		3-(2-hydroxy-5-methylbenzoyl)-5,7-dimethylpyrazolo[3,4-b]pyridine
		C16H15 N3O2 281.3		176-177 °C		Calc.: 68.31 %C; 5.37 % H; 14.94 % N Found: 68.22 %C; 5.21 % H; 14.67 % N
-------
87%
4l		3-(2-hydroxy-5-fluorobenzoyl)-5,7-dimethylpyrazolo[3,4-b]pyridine
-------		C15H12 FN3O2 285.3		160-161 °C		-------------
87%
4m		3-(2-hydroxy-5-bromobenzoyl)-5,7-dimethylpyrazolo[3,4-b]pyridine
-------		C15H12BrN3O2 346.2		150-151 °C		Calc.: 52.04 %C; 3.49 %H; 12.14 % N; 23.08 % Br Found : 52.19 %C; 3.32 %H; 12.11 % N; 23.17 % Br
89%
4n		3-(2-hydroxy-5-chlorobenzoyl)-5,7-dimethylpyrazolo[3,4-b]pyridine
-------		C15H12ClN3O2 301.7		170-171 °C		Calc.: 59.71 % C; 4.01% H; 13.93 % N; 11.75 %Cl Found: 59.58 % C; 4.16% H; 14.11 % N; 11.42 %Cl
87%
4o		3-(2-hydroxy-5-nitrobenzoyl)-5,7-dimethylpyrazolo[3,4-b] pyridine
-------		C15H12 N4O4 312.3		213-215 °C		Calc.: 57.69 % C; 3.87 %H; 17.94 % N Found : 57.53 % C; 3.68 %H; 18.11 % N
86%

The pyrazolopyridine products 4 as bifunctional compounds were used for preparation of acyl- 5, or coumarin derivatives 6. Compounds 5 were prepared from the compound 4 by a standard acylation process. 3',4'-Substituted coumarins 6 were prepared by intramolecular ring formation of compounds 5 or by condensation reaction with methylene compounds by heating for 7 - 8 hours at 140-150°C (Scheme 2). A very simple deacylation process was realized by heating of compounds 5 at 110°C in formamide (Scheme 2).

Scheme 2

The keto-groups of compounds 4 reacted with 2,4-dinitrophenylhydrazine at elevated temperature and formed hydrazones 7 (Scheme 3). Compounds 5, 6 and 7 were identified by their microanalyses and by 1H-NMR spectra.

Scheme 3

Conclusions

A simple, one pot and facile route for the preparation of 11-hydroxy-5-methyl-7-phenyl-3-(phenyloxo)pyrazolo[3,4-b]pyridine derivatives (4a-4o) from 4-oxo-4H[1]benzopyran-3-carbox-aldehydes 1 by classical and microwave irradiation heating was described. Thus, the 3-formyl-chromones 1 react with 5-amino-N-phenylpyrazole (2) under reflux in ethanol and, after opening of the pyrone ring and an intramolecular electrophilic attack of the pyrazole ring, form a new pyrazolopyridine system 4. An intermediate enamine-adduct 3 was isolated at lower temperature. Preparation of acyl derivatives 5, 3,4-substituted coumarins 6, and hydrazones 7 is also presented.

Table 2

1H-NMR spectral data of pyrazolo[3,4-b]pyridines 4

No	R	R1/8	OH	2	4	12	13	15	17(21)(2H)	18(20)(2H)	19
4a	H-14 7.63dd, J= 8.0J= 1.4	2.71s	11.86s	8.96d, J=2.1	8.46d, J = 2.1	7.13dd, J = 8.2,J = 1.1	7.57td J= 8.2,J = 1.4	7.63dd, J = 8.0J= 1.4	8.26dd J= 7.5 J=1.1	7.55t J= 7.5	7.34t J= 7.5J= 1.1
4b	CH3	2.28s2.64s	11.06s	8.94d J=1.9	8.47d J=1.9	7.04d J=7.7	7.38ddJ=7.8 J=0.8	7.39tJ=3.1 J=2.6	8.26dd,2HJ=7.6 J=1.1	7.55t,2HJ=7.6 J=1.1	7.34tJ=7.6 J=1.1
4c	F	2.72s	11.57s	8.95d J=1.9	8.45d J=1.9	7.10dd J11,12=10.2 J11,F = 4.4	7.2-7.4 m3H*13,15,19	*	8.25dd,2H J=8.0 J=1.1	7.55t,2H J=8.0 J=1.1	*
4d	Br	2.73s	11.75s	8.94d J=1.9	8.46d J=1.9	7.05d J=8.8	7.64ddJ=8.8 J=2.5	7.38t J=7.7	8.25dd,2HJ=7.7 J=1.1	7.56t,2HJ=7.7 J=1.1	7.38tJ=7.7 J=1.1
4e	Cl	2.73s	11.74s	8.95d J=2.1	8.47d J=2.1	7.10d J=9.0	7.51ddJ=9.0 J=2.5	7.60d J=2.5	8.25dd,2HJ=7.7 J=1.1	7.56t,2HJ=7.7 J=1.1	7.35tJ=8.0 J=1.1
4f	NO2	2.73s	12.60s	8.99d J=2.0	8.49d J=2.0	7.23d J=9.0	8.46ddJ=9.0 J=2.6	8.65d J=2.6	8.25dd,2HJ=7.6 J=1.1	7.56t,2HJ=7.5 J=1.1	7.55tJ=7.5 J=1.1
4g	CH3	2.28s	10.21s	8.95d	8.71d	6.92d	7.29dd	7.33d	8.48dd,2H	8.67dd,2H	---
		2.68s		J=1.9	J=1.9	J=8.2	J=8.2	J=2.2	J=9.4	J=9.4
							J=2.2		J=1.9	J=1.9
4h	F	2.67s	10.08s	8.97d	8.69d	7.05dd	7.2-7.3m	H-13,15	8.41dd,2H	8.62dd,2H
				J=2.2	J=2.2	J=8.8	2H		J=9.3	J=9.3	---
						J=4.4			J=1.1	J=1.9
4i	Br	2.67s	10.37s	8.95d	8.68d	7.00d	7.53dd	7.33d	8.41dd,2H	8.60dd,2H
				J=1.9	J=1.9	J=8.6	J=8.6	J=2.2	J=9.2	J=9.2	---
							J=2.6		J=2.0	J=2.0
4j	Cl	2.68s	10.37s	8.96d J=1.9	8.69d J=1.9	7.05d J=8.5	7.47ddJ=8.5 J=2.7	7.45d J=2.7	8.43dd,2HJ=9.3 J=2.2	8.62dd,2HJ=9.3 J=2.2	---
No	R	R1	8	OH	2	4	12	13	15
4k	CH3	2.28s2.64s	4.15s	11.67 s	8.87d J=1.9	8.40d J=1.9	7.04t J=9.1J=4.4	7.34-7.39m, (2H) 13, 15	*
4l	F	2.63s	4.15s	11.56s	8.87d J=2.0	8.39d J=2.0	7.11dd J=8.9 J=4.6	7.31td J=8.9 J=3.1J=2.6	7.29t J=3.1 J=2.6
4m	Br	2.63s	4.15s	11.74s	8.86d J=1.9	8.38d J=1.9	7.04d J=8.8	7.63dd J=8.8J=2.4	7.69d J=2.4
4n	Cl	2.65s	4.16s	11.74s	8.87d J=2.0	8.39d J=2.0	7.09d J=8.9	7.51dd J=8.9J=2.5	7.56d J=2.4
4o	NO2	2.62s	4.17s	12.50s	8.91d J=2.0	8.42d J=2.0	7.22d J=8.9	7.44dd J=8.9J=2.7	8.61d J=2.7

* multiplet

Table 3

13C-NMR: δ(ppm), J (Hz )(CDCl3)

No	R	N-CH3	C 14(CH3)	8(CH3)	2	3	4	4a
4b	CH3		20.73	12.72	150.23	127.76	130.92	116.72
4c	F			12.76	150.14	127.02	131.86	116.63
4d	Br			12.76	150.11	126.92	132.01	116.73
4e	Cl			12.76	150.11	126.91	131.96	116.57
4m	Br	33.73		12.52	149.75	125.86	132.21	114.41
No	R	5	7a	9	10	11	12	13
4b	CH3	144.48	151.5	199.04	119.31	161.37	118.74	137.93
4c	F	144.61	151.6	198.19d 4JC,F=2,5	119.11d JC,F=6.3	159.59	120.38d 3JC,F =7,4	124.45d 2JC,F=23.8
4d	Br	144.61	151.6	198.08	120.87	161.30	121.01	139.52
4e	Cl	144.61	151.6	198.14	120.2	161.84	120.62	136.72
4m	Br	142.93	151.9	198.28	120.89	162.18	120.56	139.02
No	R	14	15	16	17,21	18,20	19
4b	CH3	128.45	132.88	139.25	121.48	129.41	126.54
4c	F	155.02d 1JC,F=246.5	117.98d 2JC,F=23.8	139.11	121.54	129.45	126.68
4d	Br	110.89	135.07	139.21	121.58	129.46	126.72
4e	Cl	124.07	132.07	139.24	121.54	129.44	126.69
4m	Br	110.51	135.13	-------	--------	--------	-------

Table 4

Physical and 1H-NMR data for compounds 3,5,6,7

No-------Yield	Compound name
	Formula / MW	Melting point		Elemental analysis
	NMR data (CDCl3 unless otherwise indicated) [δ(ppm) , J (Hz )]
3a------- 72%	2-Ethyloxy-6-methyl-3-(3-methyl-1-phenylpyrazol-5-ylaminomethylene)chroman-4-one
	C23H23N3O3 383.5	164 – 166 °C		Calc.: 70.93%C; 5.95%H; 10.79%N Found: 70.90%C; 6.01%H; 10.59%N
	1H-NMR: 1.24 (t, 3H, CH3); 2.17 (s, 3H, CH3 on C-14), 2.49 (s, 3H, CH3 -6); 3.71 (q, 2H, CH2); 6.25 (s, 1H, H-2); 7.37- 7.42 (m, 3H, H-18,19,20); 7.52 (dd, 2H, 3J =8.4, 4J =2, H-17,21); 7.56 (d, 1H, 3J =8.4, H-8); 7.65 (dd, 1H, 3J =8.4, 4J =2, H-7); 7.67 (d, 1H, 4J =2, H-5); 8.08 (s, 1H, H-9); 8.67 (s, 1H, H-10); 8.99 (d, 1H, 4J =2, H-15); 9.02 (d, 1H, 4J =2.4, NH).
3b------- 61%	2-Ethyloxy-6-nitro-3-(3-methyl-1-phenylpyrazol-5-ylaminomethylene)chroman-4-one
	C22H20N4O5 420.4	187 – 189 °C		Calc.: 62.85%C; 4.75%H; 13.33%N Found: 61.40%C; 4.63%H; 13.19%N
	1H-NMR: 1.25 (t, 3H, CH3); 2.70 (s, 3H, CH3 on C-14); 3.91 (q, 2H, CH2); 6.37 (s, 1H, H-2); 7.35 (t, 1H, 3J =7.4, H-19); 7.52-758 (m, 2H, H-18,20); 8.24 (m, 2H, H-17,21); 8.35 (d, 1H , 3J =7.7, H-8); 8.42 (dd, 1H, 3J =7.7, 4J =2.7, H-7); 8.48 (d, 1H, 3J = 2.4, H-9); 8.64 (d,1H, 4J =2.7, H-5); 8.98 (d,1H,4J =2.0, H-15); 9.02 (d, 1H, 4J =2.4, NH)
5a------- 68%	3-(2-acetyloxybenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine
	C22H17N3O3 371.2	109-111 °C		Calc.: 71.12 %C; 4.58 %H, 11.31%N Found: 71.26 %C; 4.50 %H, 11.28%N
	1H-NMR: 2.03 (s, 3H, CH3CO); 2.68 (s, 3H, CH3-8); 7.30 (dd, 1H, 3J=7.3, 4J=2.1, H-13); 7.32-7.39 (m, 3H, H-11,18,20); 7.49-7.56 (m, 2H,H-10,12), 7.55 (t,1H, 3J=7.6, H-14); 8.26 (dd, 2H, 3J=7.7, 4J= 2.1, H-19,21); 8.48 (d, 1H, 4J=1.2,H-4); 9.05 (d, 1H, 4J=1.2, H-2)
5b------- 61%	3-(2-acetyloxy-5-methylbenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine
	C23H19N3O3 385.2	125-127 ° C		Calc.: 71.65 %C; 4.93 %H, 10.90%N Found: 71.44 %C; 5.09 %H, 10.81%N
	1H-NMR: 1.99 (s, 3H, CH3CO); 2.40 (s, 3H, CH3 on C-20); 2.67 (s, 3H, CH3-8); 7.11 (d, 1H, 3J=8.3, H-18); 7.30 (dd, 1H, 3J=7.3, 4J=2.1, H-19); 7.35 (d, 1H, 4J=2.2, H-21); 7.39 (m, 1H, H-14); 7.55 (t, 2H, 3J=7.6, H-10,12); 8.23 (dd, 2H, 3J=7.6, 4J=1.1, H-11,13); 8.49 (d, 1H, 4J(2,4)=1.2, H-4); 9.04 (d, 1H, 4J=1.2, H-2).
5c------ 58%	3-(2-ethyloxycarbonyloxy-5-methylbenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]-pyridine
	C24H21N3O4 415.4		154-156 ° C	Calc.: 69.39 %C; 5.09 %H; 10.11%N Found: 69.48 %C; 5.00 %H; 10.02%N
	1H-NMR:1.07 (t,3H, 3J=7,3,CH3); 2.55 (s,3H,CH3); 2.66 (s, 3H, CH3-8); 4.09 (q,2H, 3J=7,3, CH2 ); 7.23 (t, 1H, 3J=8.3, H-18); 7.37 (d, 1H, 3J=8.3, H-12); 8.09 (tt, 2H, 3J=7.6, H-11,13); 8.24 (dd, 2H, 3J=8.8, 4J=1.1, H-17,21); 8.49 (d, 1H, 4J=1.3, H-4); 9.04 (d, 1H, 4J=1.3Hz, H-2).
5d-------63%	3-[2-(3-methylphenyloxyacetyloxy-5-methyl)benzoyl]-5-methyl-7-phenylpyrazolo[3,4-b]-pyridine
	C30H25N3O4 491.3	118-120 ° C		Calc.: 73.27%C; 5.09 %H, 8.55%N Found: 73.24%C; 5.19 %H, 8.41%N
	1H-NMR: 2.41 (s, 3H, CH3); 2.25 (s, 3H, CH3); 2.64 (s, 3H, CH3-8); 4.62 (s, 2H, CH2); 6.73 (d, 1H, 4J=2.1, HPhO); 7.06-7.13 (m, 3H, HPhO); 7.3-7.4 (m, 3H, H-12,18,19); 7.45 (dd, 2H, 3J=7.3, 4J=2.1, H-11,13); 8.23 (dd, 2H, 3J=7.3, 4J=2.1, H-10,14); 8.47 (d, 1H, 4J=1.1, H-4); 9.04 (d, 1H, 4J=1.1, H-2).
6a------- 68%	3-(6'methyl-3'-phenylcoumarin-4'-yl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine
	C29H21N3O2 443.3	222-224 °C		Calc.: 78.50 %C; 4.73 %H, 9.47%N Found: 78.26 %C; 4.69 %H, 9.28%N
	1H-NMR: 2.29 (s, 3H, CH3); 2.59 (s, 3H, CH3-8); 6.95 (s, 1H, H-5'); 7.1-7.2 (m, 5H, Ph on 3'); 7.29 (t, 1H, 3J=7.3, 4J=1.9, H-12); 7.38 (dd, 2H, 3J=7.3, J=1.9, H-11,13); 7.51 (d, 1H, 3J=7.4, H-8'); 7.54 (dd, 3J=7.4, 4J=1.9, H-7'); 7.78 (d, 1H, 4J=1.2, H-4); 8.23 (dd, 2H, 3J=7.6, 4J=2.0, H-10,14); 8.39 (d, 1H, 4J=1.2, H-2).
6b------- 61%	3-[(6'methyl-3'-phenylthio)coumarin-4'-yl]-5-methyl-7-phenylpyrazolo[3,4-b]pyridine
	C29H21N3O2S 475.2	319-323 ° C		Calc.: 67.12 %C; 4.52 %H, 11.18%N Found: 66.98 %C; 4.35 %H, 11.28%N
	1H-NMR (DMSO-d6): 2.25 (s, 3H, CH3 ); 2.62 (s, 3H, CH3-8 ); 6.97 (s, 1H, H-5'); 7.1-7.2 (s, 5H, Ph on 3'); 7.33 (t, 1H, 3J=7.5, 4J=1.9, H-12); 7.48 (d,1H, 3J=8.1, H-8'); 7.52-7.55 (m, 2H, H-11,13 ); 7.56 (dd, 1H, 3J=8.3, 4J=1.9, H-7'); 8.26 (dd, 2H, 3J=8.6, 4J=2.0, H-10,14); 8.39 (d, 1H, 4J=1.2, H-4); 8.64 (d, 1H, 4J=1.2, H-2).
7a------- 63%	3-(2-hydroxybenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine-15-(2',4'-dinitrophenyl)hydrazone
	C26H19N7O5 509.5	280-281 °C		Calc.: 61.23 %C; 3.73 %H, 19.24%N Found: 61.45 %C; 3.40 %H, 19.02%N
	1H-NMR: 2.72 (s, 3H, CH3); 6.82-6.86 (m, 2H, H-19,21); 7.13 (dd, 1H, 3J=8.3Hz, 4J=2 Hz, H-18); 7.32-7.43 (m, 2H, H-12,20); 7.56 (t, 2H, 3J=7.9Hz, 4J=2Hz, H-11,13); 8.17 (d, 1H, 4J=1.6Hz, H-4); 8.27 (d, 1H, 3J=8.9Hz, H-6'); 8.28 (dd, 2H, 3J=7.9Hz, 4J=1.9Hz, H-10, 14); 8.45 (dd, 1H, 3J=9 Hz, 4J=2Hz, H-5'); 8.59 (d, 1H, 4J=1.6Hz, H-2); 9.09 (d, 1H, 4J=2Hz, H-3'); 11.26 (s, NH); 11.37 (s, OH).
7b------- 68%	3-(2-hydroxy-5-methylbenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine-15-(2', 4'-dinitrophenyl)hydrazone
	C27H21N7O5 523.5	290-292 °C		Calc.: 61.89 %C; 4.01 %H, 18.72%N Found: 61.78 %C; 4.11 %H, 18.53%N
	1H-NMR: 2.26 (s, 3H, CH3); 2.75 (s, 3H, CH3-8); 6.58 (s, 1 H, H-21); 7.05 (d, 1H, 3J=8.5Hz, H-18); 7.17 (dd, 1H, 3J=8.6Hz, 4J=2.4Hz, H-19); 7.34 (t, 1H, 3J=7.6Hz, 4J=1.1Hz, H-12); 7.55 (dd, 2H, 3J=8.5Hz, 4J=2Hz, H-11,13); 7.75 (dd, 1H,3J=8.4Hz, 4J=2.5Hz, H-6'); 8.32 (dd, 2H, 3J=8.1Hz, 4J=2Hz, H-10,14); 8.44 (dd, 1H, 3J=9.1Hz, 4J=2.6Hz, H-5'); 8.15 (d, 1H, 4J=2.8Hz, H-4); 8.57 (d, 1H, 4J=2.0, H-2); 9.07 (d, 1H, 4J=2.6 H-3'); 11.13 (s, NH); 11.20 (s, OH).
7c------- 70%	3-(2-hydroxy-5-fluorobenzoyl)-5-methyl-7-phenylpyrazolo[3,4-b]pyridine-15-(2', 4'-dinitrophenyl)hydrazone
	C26H18FN7O5 528.4	291-292 °C		-----------------
	1H-NMR: 2.66 (s, 3H, CH3); 6.94 (dd, 1H, 3J=8.8Hz, 4J=2.4Hz, H-19); 7.12 (d, 1H, 3J=8.8Hz, H-18); 7.51 (t, 1H, 3J=8.8Hz, 4J=2Hz, H-12); 7.56 (d, 1 H, 4J=2.4Hz, H-21); 8.14 (d, 1H, 4J=1.9Hz, H-4); 8.16 (dd, 2H, 3J=8.8Hz, 4J=2 Hz, H-11,13); 8.39 (dd, 1H, 3J=9.1Hz, 4J=2.6 Hz, H-5'); 8.43 (dd, 2H, 3J=8.1Hz, 4J=2Hz, H-10,14); 9.07 (d, 1H, 4J=1.9Hz, H-2); 9.09 (d, 1H, 4J=2.6 Hz, H-3'); 11.06 (s, NH); 11.27 (s, OH).