Literature DB >> 17993574

A new assay for promoter analysis in Chlamydomonas reveals roles for heat shock elements and the TATA box in HSP70A promoter-mediated activation of transgene expression.

Mukesh Lodha1, Miriam Schulz-Raffelt, Michael Schroda.   

Abstract

The aim of this work was to identify cis-regulatory sequences within the Chlamydomonas HSP70A promoter that mediate its stimulatory effect on the expression of downstream promoters. For this, we deleted/mutated the HSP70A promoter and, using a new assay, quantified its stimulatory effect. Our results indicate that the effect is mediated largely by heat shock elements and the TATA box.

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Year:  2007        PMID: 17993574      PMCID: PMC2224150          DOI: 10.1128/EC.00055-07

Source DB:  PubMed          Journal:  Eukaryot Cell        ISSN: 1535-9786


  13 in total

1.  A chloroplast-targeted heat shock protein 70 (HSP70) contributes to the photoprotection and repair of photosystem II during and after photoinhibition.

Authors:  M Schroda; O Vallon; F A Wollman; C F Beck
Journal:  Plant Cell       Date:  1999-06       Impact factor: 11.277

2.  Transcriptional enhancers--on/off gene regulation as an adaptation to silencing in higher eukaryotic nuclei.

Authors:  D I Martin
Journal:  Trends Genet       Date:  2001-08       Impact factor: 11.639

3.  Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas.

Authors:  Michael Schroda; Christoph F Beck; Olivier Vallon
Journal:  Plant J       Date:  2002-08       Impact factor: 6.417

Review 4.  Heat shock factor 1 as a coordinator of stress and developmental pathways.

Authors:  Julius Anckar; Lea Sistonen
Journal:  Adv Exp Med Biol       Date:  2007       Impact factor: 2.622

5.  The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas.

Authors:  M Schroda; D Blöcker; C F Beck
Journal:  Plant J       Date:  2000-01       Impact factor: 6.417

6.  Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas.

Authors:  H Cerutti; A M Johnson; N W Gillham; J E Boynton
Journal:  Plant Cell       Date:  1997-06       Impact factor: 11.277

7.  Expression of the arylsulphatase reporter gene under the control of the nit1 promoter in Chlamydomonas reinhardtii.

Authors:  M Ohresser; R F Matagne; R Loppes
Journal:  Curr Genet       Date:  1997-03       Impact factor: 3.886

8.  J-domain protein CDJ2 and HSP70B are a plastidic chaperone pair that interacts with vesicle-inducing protein in plastids 1.

Authors:  Cuimin Liu; Felix Willmund; Julian P Whitelegge; Susan Hawat; Bettina Knapp; Mukesh Lodha; Michael Schroda
Journal:  Mol Biol Cell       Date:  2005-01-05       Impact factor: 4.138

9.  Analysis of chromatin structure in the control regions of the chlamydomonas HSP70A and RBCS2 genes.

Authors:  Mukesh Lodha; Michael Schroda
Journal:  Plant Mol Biol       Date:  2005-10       Impact factor: 4.076

10.  Identification of a plastid response element that acts as an enhancer within the Chlamydomonas HSP70A promoter.

Authors:  Erika D von Gromoff; Michael Schroda; Ulrike Oster; Christoph F Beck
Journal:  Nucleic Acids Res       Date:  2006-09-13       Impact factor: 16.971

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  15 in total

1.  Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii.

Authors:  Lianyong Wang; Takashi Yamano; Masataka Kajikawa; Masafumi Hirono; Hideya Fukuzawa
Journal:  Photosynth Res       Date:  2014-02-19       Impact factor: 3.573

2.  Biolistic Transformation of Haematococcus pluvialis With Constructs Based on the Flanking Sequences of Its Endogenous Alpha Tubulin Gene.

Authors:  Guanhua Yuan; Xiaoying Xu; Wei Zhang; Wenlei Zhang; Yulin Cui; Song Qin; Tianzhong Liu
Journal:  Front Microbiol       Date:  2019-08-02       Impact factor: 5.640

3.  Transcription factor-dependent chromatin remodeling at heat shock and copper-responsive promoters in Chlamydomonas reinhardtii.

Authors:  Daniela Strenkert; Stefan Schmollinger; Frederik Sommer; Miriam Schulz-Raffelt; Michael Schroda
Journal:  Plant Cell       Date:  2011-06-24       Impact factor: 11.277

4.  An inducible artificial microRNA system for Chlamydomonas reinhardtii confirms a key role for heat shock factor 1 in regulating thermotolerance.

Authors:  Stefan Schmollinger; Daniela Strenkert; Michael Schroda
Journal:  Curr Genet       Date:  2010-05-07       Impact factor: 3.886

5.  A mediator of singlet oxygen responses in Chlamydomonas reinhardtii and Arabidopsis identified by a luciferase-based genetic screen in algal cells.

Authors:  Ning Shao; Guang You Duan; Ralph Bock
Journal:  Plant Cell       Date:  2013-10-22       Impact factor: 11.277

6.  Genomics of Volvocine Algae.

Authors:  James G Umen; Bradley J S C Olson
Journal:  Adv Bot Res       Date:  2012       Impact factor: 2.175

7.  Expression of the high light-inducible Dunaliella LIP promoter in Chlamydomonas reinhardtii.

Authors:  Seunghye Park; Yew Lee; Jae-Hyeok Lee; EonSeon Jin
Journal:  Planta       Date:  2013-09-17       Impact factor: 4.116

8.  Function and regulation of the glutathione peroxidase homologous gene GPXH/GPX5 in Chlamydomonas reinhardtii.

Authors:  Beat B Fischer; Régine Dayer; Yvonne Schwarzenbach; Stéphane D Lemaire; Renata Behra; Anja Liedtke; Rik I L Eggen
Journal:  Plant Mol Biol       Date:  2009-12       Impact factor: 4.076

9.  Comparative genomics in Chlamydomonas and Plasmodium identifies an ancient nuclear envelope protein family essential for sexual reproduction in protists, fungi, plants, and vertebrates.

Authors:  Jue Ning; Thomas D Otto; Claudia Pfander; Frank Schwach; Mathieu Brochet; Ellen Bushell; David Goulding; Mandy Sanders; Paul A Lefebvre; Jimin Pei; Nick V Grishin; Gary Vanderlaan; Oliver Billker; William J Snell
Journal:  Genes Dev       Date:  2013-05-15       Impact factor: 11.361

10.  Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide.

Authors:  Beth A Rasala; Philip A Lee; Zhouxin Shen; Steven P Briggs; Michael Mendez; Stephen P Mayfield
Journal:  PLoS One       Date:  2012-08-24       Impact factor: 3.240

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