Ming-Yu Li1, Zhu-Ting Xu. 1. Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, China. lmy1691@online.sh.cn
Abstract
OBJECTIVES: To search for a substance in lotus leaf that could efficiently be used in toothpaste for preventing periodontal disease. METHODS: Dried lotus leaves were hydrated and fractionated using 40% butanol and 40% ethanol extraction. The extracts and the residue were evaluated by antimicrobial sensitivity tests of broth micro-dilution methods on 96-microwell plates for inhibition of five species of oral bacteria: Actinobacillus actinomycetemcomitans Y4, Actinomyces viscosus 19246, Porphyromonas gingivalis 33277, Fusobacterium nucleatum 25586, and Actinomyces naeslundii wvl 45. The most active antimicrobial extract was then ready to use in toothpaste. A water extract of the toothpaste was prepared and its inhibitory activity tested. The potency of the lotus leaf extracts was compared to inhibition observed by three commercially available toothpastes. RESULTS: The butanol-extracted material was separated into four fractions on silica gel. Fraction #3, the fraction containing the most potent inhibitor, was formulated into toothpaste. The toothpaste containing the extract showed an inhibition effect on suspicious peridontitis-causing bacteria. CONCLUSION: An extract from the lotus leaf is effective in inhibiting peridontitis-causing bacteria. The toothpaste containing the lotus leaf-derived inhibitor could be useful in preventing periodontal disease.
OBJECTIVES: To search for a substance in lotus leaf that could efficiently be used in toothpaste for preventing periodontal disease. METHODS: Dried lotus leaves were hydrated and fractionated using 40% butanol and 40% ethanol extraction. The extracts and the residue were evaluated by antimicrobial sensitivity tests of broth micro-dilution methods on 96-microwell plates for inhibition of five species of oral bacteria: Actinobacillus actinomycetemcomitans Y4, Actinomyces viscosus 19246, Porphyromonas gingivalis 33277, Fusobacterium nucleatum 25586, and Actinomyces naeslundii wvl 45. The most active antimicrobial extract was then ready to use in toothpaste. A water extract of the toothpaste was prepared and its inhibitory activity tested. The potency of the lotus leaf extracts was compared to inhibition observed by three commercially available toothpastes. RESULTS: The butanol-extracted material was separated into four fractions on silica gel. Fraction #3, the fraction containing the most potent inhibitor, was formulated into toothpaste. The toothpaste containing the extract showed an inhibition effect on suspicious peridontitis-causing bacteria. CONCLUSION: An extract from the lotus leaf is effective in inhibiting peridontitis-causing bacteria. The toothpaste containing the lotus leaf-derived inhibitor could be useful in preventing periodontal disease.