Literature DB >> 1799226

Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic.

J M van Noort1, P van den Berg, I E Mattern.   

Abstract

A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger.

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Year:  1991        PMID： 1799226     DOI： 10.1016/0003-2697(91)90444-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  A sandwiched-culture technique for evaluation of heterologous protein production in a filamentous fungus.

Authors:  S A Asgeirsdóttir; K Scholtmeijer; J G Wessels
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

2.  Isolation and characterization of mutants of Aspergillus niger deficient in extracellular proteases.

Authors:  I E Mattern; J M van Noort; P van den Berg; D B Archer; I N Roberts; C A van den Hondel
Journal:  Mol Gen Genet       Date:  1992-08

3.  Cloning and sequencing of a serine proteinase gene from a thermophilic Bacillus species and its expression in Escherichia coli.

Authors:  B Maciver; R H McHale; D J Saul; P L Bergquist
Journal:  Appl Environ Microbiol       Date:  1994-11       Impact factor: 4.792

4.  New protease mutants in Aspergillus niger result in strongly reduced in vitro degradation of target proteins; genetical and biochemical characterization of seven complementation groups.

Authors:  J P van den Hombergh; P J van de Vondervoort; N C van der Heijden; J Visser
Journal:  Curr Genet       Date:  1995-09       Impact factor: 3.886
  4 in total

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