Literature DB >> 10224030

A sandwiched-culture technique for evaluation of heterologous protein production in a filamentous fungus.

S A Asgeirsdóttir1, K Scholtmeijer, J G Wessels.   

Abstract

Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ABH1 hydrophobin of Agaricus bisporus in A. niger, we showed that low production levels of the heterologous protein are probably due to extracellular proteolytic degradation of the protein.

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Year:  1999        PMID： 10224030      PMCID： PMC91327     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  11 in total

1.  Transformation of filamentous fungi based on hygromycin B and phleomycin resistance markers.

Authors:  P J Punt; C A van den Hondel
Journal:  Methods Enzymol       Date:  1992       Impact factor: 1.600

2.  Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.

Authors:  P J Punt; R P Oliver; M A Dingemanse; P H Pouwels; C A van den Hondel
Journal:  Gene       Date:  1987       Impact factor: 3.688

3.  Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic.

Authors:  J M van Noort; P van den Berg; I E Mattern
Journal:  Anal Biochem       Date:  1991-11-01       Impact factor: 3.365

4.  Localization of growth and secretion of proteins in Aspergillus niger.

Authors:  H A Wösten; S M Moukha; J H Sietsma; J G Wessels
Journal:  J Gen Microbiol       Date:  1991-08

5.  An abundant hydrophobin (ABH1) forms hydrophobic rodlet layers in Agaricus bisporus fruiting bodies.

Authors:  L G Lugones; J S Bosscher; K Scholtmeyer; O M de Vries; J G Wessels
Journal:  Microbiology (Reading)       Date:  1996-05       Impact factor: 2.777

Review 6.  Hydrophobins: proteins that change the nature of the fungal surface.

Authors:  J G Wessels
Journal:  Adv Microb Physiol       Date:  1997       Impact factor: 3.517

7.  Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.

Authors:  C R Merril; D Goldman; S A Sedman; M H Ebert
Journal:  Science       Date:  1981-03-27       Impact factor: 47.728

8.  Secretion of active human mucus proteinase inhibitor by Aspergillus niger after KEX2-like processing of a glucoamylase-inhibitor fusion protein.

Authors:  T Mikosch; P Klemm; H G Gassen; C A van den Hondel; M Kemme
Journal:  J Biotechnol       Date:  1996-12-10       Impact factor: 3.307

9.  Development of a homologous transformation system for Aspergillus niger based on the pyrG gene.

Authors:  W van Hartingsveldt; I E Mattern; C M van Zeijl; P H Pouwels; C A van den Hondel
Journal:  Mol Gen Genet       Date:  1987-01

10.  The Agaricus bisporus hypA gene encodes a hydrophobin and specifically accumulates in peel tissue of mushroom caps during fruit body development.

Authors:  P W De Groot; P J Schaap; A S Sonnenberg; J Visser; L J Van Griensven
Journal:  J Mol Biol       Date:  1996-04-19       Impact factor: 5.469
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  1 in total

1.  Production of the Aspergillus aculeatus endo-1,4-beta-mannanase in A. niger.

Authors:  Petrus J van Zyl; V Moodley; S H Rose; R L Roth; W H van Zyl
Journal:  J Ind Microbiol Biotechnol       Date:  2009-03-10       Impact factor: 3.346
  1 in total

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