Literature DB >> 17990881

Protonation states of buried histidine residues in human deoxyhemoglobin revealed by neutron crystallography.

Toshiyuki Chatake1, Naoya Shibayama, Sam-Yong Park, Kazuo Kurihara, Taro Tamada, Ichiro Tanaka, Nobuo Niimura, Ryota Kuroki, Yukio Morimoto.   

Abstract

The protonation states of buried histidine residues in human deoxyhemoglobin were unambiguously identified by using a neutron crystallographic technique. Unexpectedly, the neutron structure reveals that both the alpha- and beta-distal histidines (Hisalpha58 and Hisbeta63) adopt a positively charged, fully (doubly) protonated form, suggesting their contribution to the Bohr effect. In addition, the neutron data provide an accurate picture of the alpha1beta1 hydrogen-bonding network and allow us to observe unambiguously the nature of the intradimeric interactions at an atomic level.

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Year:  2007        PMID： 17990881     DOI： 10.1021/ja0749441

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  8 in total

1.  Preliminary neutron and X-ray crystallographic studies of equine cyanomethemoglobin.

Authors:  A Y Kovalevsky; S Zoe Fisher; Sean Seaver; Marat Mustyakimov; Narayanasami Sukumar; Paul Langan; Timothy C Mueser; B Leif Hanson
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-03-31

2.  Hemoglobin redux: combining neutron and X-ray diffraction with mass spectrometry to analyse the quaternary state of oxidized hemoglobins.

Authors:  Timothy C Mueser; Wendell P Griffith; Andrey Y Kovalevsky; Jingshu Guo; Sean Seaver; Paul Langan; B Leif Hanson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-10-20

3.  Protonation states of histidine and other key residues in deoxy normal human adult hemoglobin by neutron protein crystallography.

Authors:  Andrey Kovalevsky; Toshiyuki Chatake; Naoya Shibayama; Sam Yong Park; Takuya Ishikawa; Marat Mustyakimov; S Zoe Fisher; Paul Langan; Yukio Morimoto
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-10-20

4.  Macromolecular neutron crystallography at the Protein Crystallography Station (PCS).

Authors:  Andrey Kovalevsky; Zoe Fisher; Hannah Johnson; Marat Mustyakimov; Mary Jo Waltman; Paul Langan
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-10-20

Review 5.  Neutron crystallography: opportunities, challenges, and limitations.

Authors:  Matthew P Blakeley; Paul Langan; Nobuo Niimura; Alberto Podjarny
Journal:  Curr Opin Struct Biol       Date:  2008-08-07       Impact factor: 6.809

6.  Preliminary time-of-flight neutron diffraction study of human deoxyhemoglobin.

Authors:  A Y Kovalevsky; T Chatake; N Shibayama; S-Y Park; T Ishikawa; M Mustyakimov; S Z Fisher; P Langan; Y Morimoto
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-03-21

7.  Molecular modeling of the human hemoglobin-haptoglobin complex sheds light on the protective mechanisms of haptoglobin.

Authors:  Chanin Nantasenamat; Virapong Prachayasittikul; Leif Bulow
Journal:  PLoS One       Date:  2013-04-26       Impact factor: 3.240

8.  Visualizing the Bohr effect in hemoglobin: neutron structure of equine cyanomethemoglobin in the R state and comparison with human deoxyhemoglobin in the T state.

Authors:  Steven Dajnowicz; Sean Seaver; B Leif Hanson; S Zoë Fisher; Paul Langan; Andrey Y Kovalevsky; Timothy C Mueser
Journal:  Acta Crystallogr D Struct Biol       Date:  2016-06-28       Impact factor: 7.652
  8 in total

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