| Literature DB >> 17990016 |
Francisca Lúcia Lima1, Maria Auxiliadora Roque de Carvalho, Ana Carolina Morais Apolônio, Marcelo Porto Bemquerer, Marcelo Matos Santoro, Jamil Silvano Oliveira, Celuta Sales Alviano, Luiz de Macêdo Farias.
Abstract
Aggregatibacter (Actinobacillus) actinomycetemcomitans P(7-20) strain isolated from a periodontally diseased patient has produced a bacteriocin (named as actinomycetemcomitin) that is active against Peptostreptococcus anaerobius ATCC 27337. Actinomycetemcomitin was produced during exponential and stationary growth phases, and its amount decreased until it disappeared during the decline growth phase. It was purified by ammonium sulphate precipitation (30-60% saturation), and further by FPLC (mono-Q ionic exchange and Phenyl Superose hydrophobic interaction) and HPLC (C-18 reversed-phase). This bacteriocin loses its activity after incubation at a pH below 7.0 or above 8.0, following heating for 30 min at 45 degrees C, and after treatment with proteolytic enzymes such as trypsin, alpha-chymotrypsin, and papain. Actinomycetemcomitin has a molecular mass of 20.3 KDa and it represents a new bacteriocin from A. actinomycetemcomitans.Entities:
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Year: 2007 PMID： 17990016 DOI： 10.1007/s10295-007-0271-z
Source DB: PubMed Journal: J Ind Microbiol Biotechnol ISSN： 1367-5435 Impact factor: 3.346