Dirk Selmar1, Gerhard Bytof, Sven-Erik Knopp. 1. Institute for Plant Biology, Technical University Braunschweig, Mendelssohnstr. 4, 38106 Braunschweig, Germany. d.selmar@tu-bs.de
Abstract
BACKGROUND AND AIMS: When green coffee is stored for a prolonged time the coffee quality decreases distinctively. Apart from well-known 'off-notes' that arise from undesired oxidations of lipids, a typical 'flattening' of the cup quality is detectable. In order to elucidate the biological causes for this phenomenon, differentially processed coffees (wet, dry, semi-dry processing), were stored under standard conditions for 2 years and analysed comprehensively. METHODS: Wet-processed coffee was stored either as parchment coffee, where the endocarp remained around the beans or as hulled beans. Viability of coffee seeds was estimated using the tetrazolium-test of seed viability. Changes in concentration of free amino acids and soluble carbohydrates were analysed by HPLC. KEY RESULTS: Whereas all other coffees lost viability within the first 6 months of storage, coffee beans stored within the parchment remained viable for >1 year. Glucose and fructose decreased slightly in the course of storage and glutamine content declined significantly. However, the changes observed in sugar and amino acid content were not correlated with the viability of the coffee beans. Consequently, neither typical metabolic reactions occurring within living cells nor characteristic post-mortem reactions could be responsible for the observed changes. As a result of post-mortem reactions in re-imbibed seeds, a characteristic bluish-green colour developed, putatively due to the oxidation of chlorogenic acids and subsequent reactions with primary amino compounds. This coloration might be an appropriate marker to substantiate if coffee seeds had been stored for an expanded time and putative quality losses were not relevant so far. CONCLUSIONS: It is suggested that loss of viability is relevant for the aroma flattening. As neither metabolic nor post-mortem reactions were responsible for the observed changes, it is concluded that Maillard reactions that occur during storage might be the cause of the decrease in potential aroma precursors.
BACKGROUND AND AIMS: When green coffee is stored for a prolonged time the coffee quality decreases distinctively. Apart from well-known 'off-notes' that arise from undesired oxidations of lipids, a typical 'flattening' of the cup quality is detectable. In order to elucidate the biological causes for this phenomenon, differentially processed coffees (wet, dry, semi-dry processing), were stored under standard conditions for 2 years and analysed comprehensively. METHODS: Wet-processed coffee was stored either as parchment coffee, where the endocarp remained around the beans or as hulled beans. Viability of coffee seeds was estimated using the tetrazolium-test of seed viability. Changes in concentration of free amino acids and soluble carbohydrates were analysed by HPLC. KEY RESULTS: Whereas all other coffees lost viability within the first 6 months of storage, coffee beans stored within the parchment remained viable for >1 year. Glucose and fructose decreased slightly in the course of storage and glutamine content declined significantly. However, the changes observed in sugar and amino acid content were not correlated with the viability of the coffee beans. Consequently, neither typical metabolic reactions occurring within living cells nor characteristic post-mortem reactions could be responsible for the observed changes. As a result of post-mortem reactions in re-imbibed seeds, a characteristic bluish-green colour developed, putatively due to the oxidation of chlorogenic acids and subsequent reactions with primary amino compounds. This coloration might be an appropriate marker to substantiate if coffee seeds had been stored for an expanded time and putative quality losses were not relevant so far. CONCLUSIONS: It is suggested that loss of viability is relevant for the aroma flattening. As neither metabolic nor post-mortem reactions were responsible for the observed changes, it is concluded that Maillard reactions that occur during storage might be the cause of the decrease in potential aroma precursors.
Authors: Florac De Bruyn; Sophia Jiyuan Zhang; Vasileios Pothakos; Julio Torres; Charles Lambot; Alice V Moroni; Michael Callanan; Wilbert Sybesma; Stefan Weckx; Luc De Vuyst Journal: Appl Environ Microbiol Date: 2016-12-15 Impact factor: 4.792
Authors: Gustavo C Sant'Ana; Luiz F P Pereira; David Pot; Suzana T Ivamoto; Douglas S Domingues; Rafaelle V Ferreira; Natalia F Pagiatto; Bruna S R da Silva; Lívia M Nogueira; Cintia S G Kitzberger; Maria B S Scholz; Fernanda F de Oliveira; Gustavo H Sera; Lilian Padilha; Jean-Pierre Labouisse; Romain Guyot; Pierre Charmetant; Thierry Leroy Journal: Sci Rep Date: 2018-01-11 Impact factor: 4.379