Literature DB >> 1797950

An enzyme-coupled assay for acyl-CoA synthetase.

K Ichihara1, Y Shibasaki.   

Abstract

An enzyme-coupled, colorimetric method for the assay of acyl-CoA synthetase is described. Acyl-CoA formed from fatty acid and CoA by acyl-CoA synthetase was dehydrogenated by acyl-CoA oxidase. Hydrogen peroxide produced was then converted into formaldehyde in the presence of methanol by catalase. The formaldehyde reacted with a triazole compound, 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole, in an alkaline condition to form a purple dye, and the absorbance was measured spectrophotometrically.

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Year:  1991        PMID： 1797950

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  10 in total

1.  Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax deposition in rice.

Authors:  Xiaoyi Zhu; Lizhong Xiong
Journal:  Proc Natl Acad Sci U S A       Date:  2013-10-14       Impact factor: 11.205

2.  Expression of Physaria longchain acyl-CoA synthetases and hydroxy fatty acid accumulation in transgenic Arabidopsis.

Authors:  Jesse D Bengtsson; James G Wallis; John Browse
Journal:  J Plant Physiol       Date:  2022-05-11       Impact factor: 3.686

3.  The acyl-CoA synthetase encoded by LACS2 is essential for normal cuticle development in Arabidopsis.

Authors:  Judy Schnurr; Jay Shockey; John Browse
Journal:  Plant Cell       Date:  2004-02-18       Impact factor: 11.277

4.  Multiple FadD acyl-CoA synthetases contribute to differential fatty acid degradation and virulence in Pseudomonas aeruginosa.

Authors:  Yun Kang; Jan Zarzycki-Siek; Chad B Walton; Michael H Norris; Tung T Hoang
Journal:  PLoS One       Date:  2010-10-21       Impact factor: 3.240

5.  Lipid synthesis and acyl-CoA synthetase in developing rice seeds.

Authors:  Ken'ichi Ichihara; Noriaki Kobayashi; Kazumi Saito
Journal:  Lipids       Date:  2003-08       Impact factor: 1.880

6.  Dietary oligofructose lowers triglycerides, phospholipids and cholesterol in serum and very low density lipoproteins of rats.

Authors:  M Fiordaliso; N Kok; J P Desager; F Goethals; D Deboyser; M Roberfroid; N Delzenne
Journal:  Lipids       Date:  1995-02       Impact factor: 1.880

Review 7.  Mammalian long-chain acyl-CoA synthetases.

Authors:  Eric Soupene; Frans A Kuypers
Journal:  Exp Biol Med (Maywood)       Date:  2008-03-28

8.  Amelioration of Cryptosporidium parvum infection in vitro and in vivo by targeting parasite fatty acyl-coenzyme A synthetases.

Authors:  Fengguang Guo; Haili Zhang; Jason M Fritzler; S Dean Rider; Lixin Xiang; Nina N McNair; Jan R Mead; Guan Zhu
Journal:  J Infect Dis       Date:  2013-11-23       Impact factor: 5.226

9.  A Futile Metabolic Cycle of Fatty Acyl-CoA Hydrolysis and Resynthesis in Corynebacterium glutamicum and Its Disruption Leading to Fatty Acid Production.

Authors:  Masato Ikeda; Keisuke Takahashi; Tatsunori Ohtake; Ryosuke Imoto; Haruka Kawakami; Mikiro Hayashi; Seiki Takeno
Journal:  Appl Environ Microbiol       Date:  2020-12-11       Impact factor: 4.792

10.  Whole-Genome Sequencing Analysis of Quorum Quenching Bacterial Strain Acinetobacter lactucae QL-1 Identifies the FadY Enzyme for Degradation of the Diffusible Signal Factor.

Authors:  Tian Ye; Tian Zhou; Xudan Xu; Wenping Zhang; Xinghui Fan; Sandhya Mishra; Lianhui Zhang; Xiaofan Zhou; Shaohua Chen
Journal:  Int J Mol Sci       Date:  2020-09-14       Impact factor: 5.923
  10 in total

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