Literature DB >> 17952662

Molecular characterization of Kluyveromyces marxianus strains isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico.

Daisy Pérez-Brito1, Raúl Tapia-Tussell, Andrés Quijano-Ramayo, Alfonso Larqué-Saavedra, Patricia Lappe.   

Abstract

The molecular characterization of 14 strains of Kluyveromyces marxianus isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico, was performed by AP-PCR analysis, PCR-RFLP of 5.8S-ITS, and complete NTS regions. A sequence analysis of the D1/D2 domain of the 26S rDNA was also carried out in six selected strains. The AP-PCR approach had the highest discrimination power for the molecular characterization of new henequen K. marxianus strains. PCR-RFLP of 5.8S-ITS regions did not reveal polymorphisms in this group of strains. The restriction enzyme digestion analysis of NTS region enables the separation among strains which coincides with ascospore shape groups. The molecular tools used in this article may be useful to confirm a preliminary screen of yeasts isolated from henequen without the use of growth characteristics or morpho-physiological tests.

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Year:  2007        PMID： 17952662     DOI： 10.1007/s12033-007-0036-y

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  12 in total

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5.  A rapid and simple method for DNA extraction from yeasts and fungi isolated from Agave fourcroydes.

Authors:  Raul Tapia-Tussell; Patricia Lappe; Miguel Ulloa; Andrés Quijano-Ramayo; Mirbella Cáceres-Farfán; Alfonso Larqué-Saavedra; Daisy Perez-Brito
Journal:  Mol Biotechnol       Date:  2006-05       Impact factor: 2.695

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  3 in total

1.  Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting.

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Journal:  J Microbiol       Date:  2015-01-04       Impact factor: 3.422

2.  Hydrolysis of Agave fourcroydes Lemaire (henequen) leaf juice and fermentation with Kluyveromyces marxianus for ethanol production.

Authors:  Pablo A Villegas-Silva; Tanit Toledano-Thompson; Blondy B Canto-Canché; Alfonso Larqué-Saavedra; Luis F Barahona-Pérez
Journal:  BMC Biotechnol       Date:  2014-02-14       Impact factor: 2.563

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