Literature DB >> 16691008

A rapid and simple method for DNA extraction from yeasts and fungi isolated from Agave fourcroydes.

Raul Tapia-Tussell1, Patricia Lappe, Miguel Ulloa, Andrés Quijano-Ramayo, Mirbella Cáceres-Farfán, Alfonso Larqué-Saavedra, Daisy Perez-Brito.   

Abstract

A simple and easy protocol for extracting high-quality DNA from different yeast and filamentous fungal species is described. This method involves two important steps: first, the disruption of cell walls by mechanical means and freezing; and second, the extraction, isolation, and precipitation of genomic DNA. The absorbance ratios (A(260)/A(280)) obtained ranged from 1.6 to 2.0. The main objective of this procedure is to extract pure DNA from yeast and filamentous fungi, including those with high contents of proteins, polysaccharides, and other complex compounds in their cell walls. The yield and quality of the DNAs obtained were suitable for micro/minisatellite primer-polymerase chain reaction (MSP-PCR) fingerprinting as well as for the sequence of the D1/D2 domain of the 26S rDNA.

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Year:  2006        PMID： 16691008     DOI： 10.1385/MB:33:1:67

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  5 in total

1.  A fast, simple, and reliable high-yielding method for DNA extraction from different plant species.

Authors:  Raul Tapia-Tussell; Andres Quijano-Ramayo; Rafael Rojas-Herrera; Alfonso Larque-Saavedra; Daisy Perez-Brito
Journal:  Mol Biotechnol       Date:  2005-10       Impact factor: 2.695

2.  Polyphasic taxonomy of the basidiomycetous yeast genus Rhodotorula: Rh. glutinis sensu stricto and Rh. dairenensis comb. nov.

Authors:  Mário Gadanho; José Paulo Sampaio
Journal:  FEMS Yeast Res       Date:  2002-03       Impact factor: 2.796

3.  Phenotypic and genotypic identification of yeasts from cheese.

Authors:  H Prillinger; O Molnár; F Eliskases-Lechner; K Lopandic
Journal:  Antonie Van Leeuwenhoek       Date:  1999-05       Impact factor: 2.271

4.  Molecular characterization of carotenogenic yeasts from aquatic environments in Patagonia, Argentina.

Authors:  Diego Libkind; Silvia Brizzio; Alejandra Ruffini; Mário Gadanho; Maria van Broock; José Paulo Sampaio
Journal:  Antonie Van Leeuwenhoek       Date:  2003       Impact factor: 2.271

5.  Rapid isolation of yeast genomic DNA: Bust n' Grab.

Authors:  Susanna Harju; Halyna Fedosyuk; Kenneth R Peterson
Journal:  BMC Biotechnol       Date:  2004-04-21       Impact factor: 2.563
  5 in total
  14 in total

1.  Identification of predominant yeasts associated with artisan Mexican cocoa fermentations using culture-dependent and culture-independent approaches.

Authors:  A Arana-Sánchez; L E Segura-García; M Kirchmayr; I Orozco-Ávila; E Lugo-Cervantes; A Gschaedler-Mathis
Journal:  World J Microbiol Biotechnol       Date:  2015-01-08       Impact factor: 3.312

2.  Bioconversion of Agave tequilana fructans by exo-inulinases from indigenous Aspergillus niger CH-A-2010 enhances ethanol production from raw Agave tequilana juice.

Authors:  Carlos Huitrón; Rosalba Pérez; Luís Gutiérrez; Patricia Lappe; Pavel Petrosyan; Jesús Villegas; Cecilia Aguilar; Leticia Rocha-Zavaleta; Abel Blancas
Journal:  J Ind Microbiol Biotechnol       Date:  2012-11-17       Impact factor: 3.346

3.  Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting.

Authors:  Daisy Pérez-Brito; Anuar Magaña-Alvarez; Patricia Lappe-Oliveras; Alberto Cortes-Velazquez; Claudia Torres-Calzada; Teófilo Herrera-Suarez; Alfonso Larqué-Saavedra; Raul Tapia-Tussell
Journal:  J Microbiol       Date:  2015-01-04       Impact factor: 3.422

4.  A species-specific polymerase chain reaction assay for rapid and sensitive detection of Colletotrichum capsici.

Authors:  C Torres-Calzada; R Tapia-Tussell; A Quijano-Ramayo; R Martin-Mex; R Rojas-Herrera; I Higuera-Ciapara; D Perez-Brito
Journal:  Mol Biotechnol       Date:  2011-09       Impact factor: 2.695

5.  Genetic variation of Colletotrichum magnum isolated from Carica papaya as revealed by DNA fingerprinting.

Authors:  Daisy Pérez-Brito; Alberto Cortes-Velázquez; Teresita Valencia-Yah; Anuar Magaña-Álvarez; Cuauhtémoc Navarro; Blanca Moreno; Steven Quiroga; Raúl Tapia-Tussell
Journal:  J Microbiol       Date:  2018-10-24       Impact factor: 3.422

6.  Molecular characterization of Kluyveromyces marxianus strains isolated from Agave fourcroydes (Lem.) in Yucatan, Mexico.

Authors:  Daisy Pérez-Brito; Raúl Tapia-Tussell; Andrés Quijano-Ramayo; Alfonso Larqué-Saavedra; Patricia Lappe
Journal:  Mol Biotechnol       Date:  2007-07-10       Impact factor: 2.695

7.  PCR-based detection and characterization of the fungal pathogens Colletotrichum gloeosporioides and Colletotrichum capsici causing anthracnose in papaya (Carica papaya l.) in the Yucatan peninsula.

Authors:  Raul Tapia-Tussell; Andres Quijano-Ramayo; Alberto Cortes-Velazquez; Patricia Lappe; Alfonso Larque-Saavedra; Daisy Perez-Brito
Journal:  Mol Biotechnol       Date:  2008-08-01       Impact factor: 2.695

8.  Sequence and structural investigation of a novel psychrophilic α-amylase from Glaciozyma antarctica PI12 for cold-adaptation analysis.

Authors:  Aizi Nor Mazila Ramli; Mohd Akmal Azhar; Mohd Shahir Shamsir; Amir Rabu; Abdul Munir Abdul Murad; Nor Muhammad Mahadi; Rosli Md Illias
Journal:  J Mol Model       Date:  2013-05-18       Impact factor: 1.810

9.  Characterization of Two Fusarium solani Species Complex Isolates from the Ambrosia Beetle Xylosandrus morigerus.

Authors:  Nohemí Carreras-Villaseñor; José B Rodríguez-Haas; Luis A Martínez-Rodríguez; Alan J Pérez-Lira; Enrique Ibarra-Laclette; Emanuel Villafán; Ana P Castillo-Díaz; Luis A Ibarra-Juárez; Edgar D Carrillo-Hernández; Diana Sánchez-Rangel
Journal:  J Fungi (Basel)       Date:  2022-02-26

10.  Draft Genome Sequence of the Phytopathogenic Fungus Fusarium euwallaceae, the Causal Agent of Fusarium Dieback.

Authors:  Enrique Ibarra-Laclette; Diana Sánchez-Rangel; Eric Hernández-Domínguez; Claudia-Anahí Pérez-Torres; Randy Ortiz-Castro; Emanuel Villafán; Alexandro Alonso-Sánchez; Benjamín Rodríguez-Haas; Abel López-Buenfil; Clemente García-Avila; José-Abrahán Ramírez-Pool
Journal:  Genome Announc       Date:  2017-08-31
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