Literature DB >> 17951757

Nuclear magnetic resonance structural studies of membrane proteins in micelles and bilayers.

Xiao-Min Gong1, Carla M Franzin, Khang Thai, Jinghua Yu, Francesca M Marassi.   

Abstract

Nuclear magnetic resonance (NMR) spectroscopy enables determination of membrane protein structures in lipid environments, such as micelles and bilayers. This chapter outlines the steps for membrane-protein structure determination using solution NMR with micelle samples, and solid-state NMR with oriented lipid-bilayer samples. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from gamma and CHIF, two membrane proteins that function as regulatory subunits of the Na+- and K+-ATPase.

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Year:  2007        PMID： 17951757      PMCID： PMC2918260          DOI： 10.1007/978-1-59745-519-0_35

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  51 in total

1.  High-resolution conformation of gramicidin A in a lipid bilayer by solid-state NMR.

Authors:  R R Ketchem; W Hu; T A Cross
Journal:  Science       Date:  1993-09-10       Impact factor: 47.728

2.  Use of T7 RNA polymerase to direct expression of cloned genes.

Authors:  F W Studier; A H Rosenberg; J J Dunn; J W Dubendorff
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

3.  Purification of Na+,K+-ATPase: enzyme sources, preparative problems, and preparation from mammalian kidney.

Authors:  P L Jørgensen
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

4.  Analysis of Na+,K+-ATPase by electron microscopy.

Authors:  A B Maunsbach; E Skriver; P L Jørgensen
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

Review 5.  Chemical shifts as a tool for structure determination.

Authors:  D S Wishart; B D Sykes
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

6.  A heteronuclear correlation experiment for simultaneous determination of 15N longitudinal decay and chemical exchange rates of systems in slow equilibrium.

Authors:  N A Farrow; O Zhang; J D Forman-Kay; L E Kay
Journal:  J Biomol NMR       Date:  1994-09       Impact factor: 2.835

7.  Localization of the affinity peptide-substrate inactivator site on recombinant vitamin K-dependent carboxylase.

Authors:  A Kuliopulos; N P Nelson; M Yamada; C T Walsh; B Furie; B C Furie; D A Roth
Journal:  J Biol Chem       Date:  1994-08-19       Impact factor: 5.157

8.  Formation of a native-like subdomain in a partially folded intermediate of bovine pancreatic trypsin inhibitor.

Authors:  J P Staley; P S Kim
Journal:  Protein Sci       Date:  1994-10       Impact factor: 6.725

9.  A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.

Authors:  M Ikura; L E Kay; A Bax
Journal:  Biochemistry       Date:  1990-05-15       Impact factor: 3.162

10.  Molecular cloning and immunological characterization of the gamma polypeptide, a small protein associated with the Na,K-ATPase.

Authors:  R W Mercer; D Biemesderfer; D P Bliss; J H Collins; B Forbush
Journal:  J Cell Biol       Date:  1993-05       Impact factor: 10.539
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  11 in total

1.  Tilt and azimuthal angles of a transmembrane peptide: a comparison between molecular dynamics calculations and solid-state NMR data of sarcolipin in lipid membranes.

Authors:  Lei Shi; Alessandro Cembran; Jiali Gao; Gianluigi Veglia
Journal:  Biophys J       Date:  2009-05-06       Impact factor: 4.033

2.  Estimation of relative order tensors, and reconstruction of vectors in space using unassigned RDC data and its application.

Authors:  Xijiang Miao; Rishi Mukhopadhyay; Homayoun Valafar
Journal:  J Magn Reson       Date:  2008-07-12       Impact factor: 2.229

3.  NMR studies of membrane proteins.

Authors:  Rob Kaptein; Gerhard Wagner
Journal:  J Biomol NMR       Date:  2015-04       Impact factor: 2.835

4.  A systematic assessment of mature MBP in membrane protein production: overexpression, membrane targeting and purification.

Authors:  Jian Hu; Huajun Qin; Fei Philip Gao; Timothy A Cross
Journal:  Protein Expr Purif       Date:  2011-06-13       Impact factor: 1.650

Review 5.  Lipid raft redox signaling: molecular mechanisms in health and disease.

Authors:  Si Jin; Fan Zhou; Foad Katirai; Pin-Lan Li
Journal:  Antioxid Redox Signal       Date:  2011-05-11       Impact factor: 8.401

6.  A refinement protocol to determine structure, topology, and depth of insertion of membrane proteins using hybrid solution and solid-state NMR restraints.

Authors:  Lei Shi; Nathaniel J Traaseth; Raffaello Verardi; Alessandro Cembran; Jiali Gao; Gianluigi Veglia
Journal:  J Biomol NMR       Date:  2009-07-14       Impact factor: 2.835

7.  Backbone structure of a small helical integral membrane protein: A unique structural characterization.

Authors:  Richard C Page; Sangwon Lee; Jacob D Moore; Stanley J Opella; Timothy A Cross
Journal:  Protein Sci       Date:  2009-01       Impact factor: 6.725

Review 8.  G-protein-coupled receptor structures were not built in a day.

Authors:  Tracy M Blois; James U Bowie
Journal:  Protein Sci       Date:  2009-07       Impact factor: 6.725

9.  Structural biology of human cannabinoid receptor-2 helix 6 in membrane-mimetic environments.

Authors:  Elvis K Tiburu; Sergiy Tyukhtenko; Lalit Deshmukh; Olga Vinogradova; David R Janero; Alexandros Makriyannis
Journal:  Biochem Biophys Res Commun       Date:  2009-05-03       Impact factor: 3.575

10.  Efficient and accurate estimation of relative order tensors from lambda-maps.

Authors:  Rishi Mukhopadhyay; Xijiang Miao; Paul Shealy; Homayoun Valafar
Journal:  J Magn Reson       Date:  2009-03-05       Impact factor: 2.229
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