Literature DB >> 19397896

Structural biology of human cannabinoid receptor-2 helix 6 in membrane-mimetic environments.

Elvis K Tiburu1, Sergiy Tyukhtenko, Lalit Deshmukh, Olga Vinogradova, David R Janero, Alexandros Makriyannis.   

Abstract

We detail the structure and dynamics of a synthetic peptide corresponding to transmembrane helix 6 (TMH6) of human cannabinoid receptor-2 (hCB2) in biomembrane-mimetic environments. The peptide's NMR structural biology is characterized by two alpha-helical domains bridged by a flexible, nonhelical hinge region containing a highly-conserved CWFP motif with an environmentally sensitive, Pro-based conformational switch. Buried within the peptide's flexible region, W(258) may hydrogen-bond with L(255) to help stabilize the Pro-kinked hCB2 TMH6 structure and position C(257) advantageously for interaction with agonist ligands. These characteristics of hCB2 TMH6 are potential structural features of ligand-induced hCB2 activation in vivo.

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Year:  2009        PMID： 19397896      PMCID： PMC3679894          DOI： 10.1016/j.bbrc.2009.04.099

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  40 in total

1.  (-)-7'-Isothiocyanato-11-hydroxy-1',1'-dimethylheptylhexahydrocannabinol (AM841), a high-affinity electrophilic ligand, interacts covalently with a cysteine in helix six and activates the CB1 cannabinoid receptor.

Authors:  Robert P Picone; Atmaram D Khanolkar; Wei Xu; Lionel A Ayotte; Ganesh A Thakur; Dow P Hurst; Mary E Abood; Patricia H Reggio; Donna J Fournier; Alexandros Makriyannis
Journal:  Mol Pharmacol       Date:  2005-09-12       Impact factor: 4.436

2.  Structure and dynamics of the second and third transmembrane domains of human glycine receptor.

Authors:  Dejian Ma; Zhanwu Liu; Ling Li; Pei Tang; Yan Xu
Journal:  Biochemistry       Date:  2005-06-21       Impact factor: 3.162

3.  Homology models of the cannabinoid CB1 and CB2 receptors. A docking analysis study.

Authors:  Cristina Montero; Nuria Eugenia Campillo; Pilar Goya; Juan Antonio Páez
Journal:  Eur J Med Chem       Date:  2005-01       Impact factor: 6.514

Review 4.  The pharmacology of cannabinoid receptors and their ligands: an overview.

Authors:  R G Pertwee
Journal:  Int J Obes (Lond)       Date:  2006-04       Impact factor: 5.095

5.  Solution structure of YKR049C, a putative redox protein from Saccharomyces cerevisiae.

Authors:  Jin-Won Jung; Adelinda Yee; Bin Wu; Cheryl H Arrowsmith; Weontae Lee
Journal:  J Biochem Mol Biol       Date:  2005-09-30

6.  Circular dichroic analysis of protein conformation: inclusion of the beta-turns.

Authors:  C T Chang; C S Wu; J T Yang
Journal:  Anal Biochem       Date:  1978-11       Impact factor: 3.365

7.  Crystallography & NMR system: A new software suite for macromolecular structure determination.

Authors:  A T Brünger; P D Adams; G M Clore; W L DeLano; P Gros; R W Grosse-Kunstleve; J S Jiang; J Kuszewski; M Nilges; N S Pannu; R J Read; L M Rice; T Simonson; G L Warren
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1998-09-01

8.  Non-random distribution of amino acids in the transmembrane segments of human type I single span membrane proteins.

Authors:  C Landolt-Marticorena; K A Williams; C M Deber; R A Reithmeier
Journal:  J Mol Biol       Date:  1993-02-05       Impact factor: 5.469

9.  Main-chain bond lengths and bond angles in protein structures.

Authors:  R A Laskowski; D S Moss; J M Thornton
Journal:  J Mol Biol       Date:  1993-06-20       Impact factor: 5.469

Review 10.  Trifluoroethanol and colleagues: cosolvents come of age. Recent studies with peptides and proteins.

Authors:  M Buck
Journal:  Q Rev Biophys       Date:  1998-08       Impact factor: 5.318
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  8 in total

1.  hCB2 ligand-interaction landscape: cysteine residues critical to biarylpyrazole antagonist binding motif and receptor modulation.

Authors:  Richard W Mercier; Ying Pei; Lakshmipathi Pandarinathan; David R Janero; Jing Zhang; Alexandros Makriyannis
Journal:  Chem Biol       Date:  2010-10-29

2.  Mass spectrometry-based proteomics of human cannabinoid receptor 2: covalent cysteine 6.47(257)-ligand interaction affording megagonist receptor activation.

Authors:  Dennis W Szymanski; Malvina Papanastasiou; Katja Melchior; Nikolai Zvonok; Richard W Mercier; David R Janero; Ganesh A Thakur; Sangwon Cha; Billy Wu; Barry Karger; Alexandros Makriyannis
Journal:  J Proteome Res       Date:  2011-09-13       Impact factor: 4.466

3.  NMR solution structure of human cannabinoid receptor-1 helix 7/8 peptide: candidate electrostatic interactions and microdomain formation.

Authors:  Sergiy Tyukhtenko; Elvis K Tiburu; Lalit Deshmukh; Olga Vinogradova; David R Janero; Alexandros Makriyannis
Journal:  Biochem Biophys Res Commun       Date:  2009-09-18       Impact factor: 3.575

4.  Large multiple transmembrane domain fragments of a G protein-coupled receptor: biosynthesis, purification, and biophysical studies.

Authors:  Zhanna Potetinova; Subramanyam Tantry; Leah S Cohen; Katrina E Caroccia; Boris Arshava; Jeffrey M Becker; Fred Naider
Journal:  Biopolymers       Date:  2012       Impact factor: 2.505

5.  Crystal structures of agonist-bound human cannabinoid receptor CB1.

Authors:  Tian Hua; Kiran Vemuri; Spyros P Nikas; Robert B Laprairie; Yiran Wu; Lu Qu; Mengchen Pu; Anisha Korde; Shan Jiang; Jo-Hao Ho; Gye Won Han; Kang Ding; Xuanxuan Li; Haiguang Liu; Michael A Hanson; Suwen Zhao; Laura M Bohn; Alexandros Makriyannis; Raymond C Stevens; Zhi-Jie Liu
Journal:  Nature       Date:  2017-07-05       Impact factor: 49.962

6.  Modeling, molecular dynamics simulation, and mutation validation for structure of cannabinoid receptor 2 based on known crystal structures of GPCRs.

Authors:  Zhiwei Feng; Mohammed Hamed Alqarni; Peng Yang; Qin Tong; Ananda Chowdhury; Lirong Wang; Xiang-Qun Xie
Journal:  J Chem Inf Model       Date:  2014-09-05       Impact factor: 4.956

7.  Expression, Purification, and Monitoring of Conformational Changes of hCB2 TMH67H8 in Different Membrane-Mimetic Lipid Mixtures Using Circular Dichroism and NMR Techniques.

Authors:  Elvis K Tiburu; Jianqin Zhuang; Heidimarie N A Fleischer; Patrick K Arthur; Gordon A Awandare
Journal:  Membranes (Basel)       Date:  2017-02-17

Review 8.  Challenges and Perspectives in Chemical Synthesis of Highly Hydrophobic Peptides.

Authors:  Lena K Mueller; Andreas C Baumruck; Hanna Zhdanova; Alesia A Tietze
Journal:  Front Bioeng Biotechnol       Date:  2020-03-04
  8 in total

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