Literature DB >> 1793585

Electric measurements can be used to monitor the attachment and spreading of cells in tissue culture.

P Mitra1, C R Keese, I Giaever.   

Abstract

A new electrical assay to measure the attachment and spreading of cells in tissue culture has been developed and substantiated by comparison with a more conventional assay. Small gold electrodes are vacuum deposited on the bottom of standard polystyrene culture dishes and coated with various proteins. As mammalian fibroblasts attach and spread on these surfaces, the measured impedance of the electrodes changes. These impedance changes reflect the amount of area blocked by the spreading cells. Since the weak electrical signals used have no noticeable effects on the cells, this is a very convenient method that is both quantitative and sensitive for measuring cell attachment and spreading.

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Year:  1991        PMID： 1793585

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  19 in total

1.  Altered impedance during pigment aggregation in Xenopus laevis melanophores.

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2.  Impedance-based monitoring of ongoing cardiomyocyte death induced by tumor necrosis factor-alpha.

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4.  Monitoring electropermeabilization in the plasma membrane of adherent mammalian cells.

Authors:  P M Ghosh; C R Keese; I Giaever
Journal:  Biophys J       Date:  1993-05       Impact factor: 4.033

5.  Rac1 acts in conjunction with Nedd4 and dishevelled-1 to promote maturation of cell-cell contacts.

Authors:  Micha Nethe; Bart-Jan de Kreuk; Daniele V F Tauriello; Eloise C Anthony; Barbara Snoek; Thomas Stumpel; Patricia C Salinas; Madelon M Maurice; Dirk Geerts; André M Deelder; Paul J Hensbergen; Peter L Hordijk
Journal:  J Cell Sci       Date:  2012-03-30       Impact factor: 5.285

6.  Use of electric cell-substrate impedance sensing to assess in vitro cytotoxicity.

Authors:  Daniel Opp; Brian Wafula; Jennifer Lim; Eric Huang; Jun-Chih Lo; Chun-Min Lo
Journal:  Biosens Bioelectron       Date:  2009-01-23       Impact factor: 10.618

7.  Prostaglandin E2 elicits a morphological change in cultured orbital fibroblasts from patients with Graves ophthalmopathy.

Authors:  T J Smith; H S Wang; M G Hogg; R C Henrikson; C R Keese; I Giaever
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-24       Impact factor: 11.205

8.  Towards an Automated MEMS-based Characterization of Benign and Cancerous Breast Tissue using Bioimpedance Measurements.

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Journal:  Sens Actuators B Chem       Date:  2014-08-01       Impact factor: 7.460

9.  Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function.

Authors:  C Tiruppathi; A B Malik; P J Del Vecchio; C R Keese; I Giaever
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-01       Impact factor: 11.205

10.  Pericytes regulate vascular basement membrane remodeling and govern neutrophil extravasation during inflammation.

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Journal:  PLoS One       Date:  2012-09-21       Impact factor: 3.240
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